Rapid Identification of Bio-Molecules Applied for Detection of Biosecurity Agents Using Rolling Circle Amplification

被引：40

作者：

Goransson, Jenny ^{[1
,3
]}

Ke, Rongqin ^{[1
]}

Nong, Rachel Yuan ^{[1
]}

Howell, W. Mathias ^{[1
]}

Karman, Anna ^{[2
,3
]}

Grawe, Jan ^{[3
]}

Stenberg, Johan ^{[3
]}

Granberg, Malin ^{[4
]}

Elgh, Magnus ^{[3
]}

Herthnek, David ^{[1
]}

Wikstrom, Per ^{[4
]}

Jarvius, Jonas ^{[1
,3
]}

Nilsson, Mats ^{[1
]}

机构：

[1] Uppsala Univ, SciLifeLab, Dept Immunol Genet & Pathol, Rudbeck Lab, Uppsala, Sweden

[2] Dag Hammarskjolds Vag, Olink AB, Uppsala, Sweden

[3] Dag Hammarskjolds, Q Linea AB, Uppsala, Sweden

[4] Swedish Def Res Agcy, Umea, Sweden

来源：

PLOS ONE | 2012年 / 7卷 / 02期

基金：

瑞典研究理事会;

关键词：

BACILLUS-ANTHRACIS; FRANCISELLA-TULARENSIS; PROXIMITY LIGATION; MAGNETIC BIOSENSOR; PROTEIN-DETECTION; YERSINIA-PESTIS; PADLOCK PROBES; IN-SITU; ASSAYS; PCR;

D O I：

10.1371/journal.pone.0031068

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Detection and identification of pathogens in environmental samples for biosecurity applications are challenging due to the strict requirements on specificity, sensitivity and time. We have developed a concept for quick, specific and sensitive pathogen identification in environmental samples. Target identification is realized by padlock- and proximity probing, and reacted probes are amplified by RCA (rolling-circle amplification). The individual RCA products are labeled by fluorescence and enumerated by an instrument, developed for sensitive and rapid digital analysis. The concept is demonstrated by identification of simili biowarfare agents for bacteria (Escherichia coli and Pantoea agglomerans) and spores (Bacillus atrophaeus) released in field.

引用

页数：9

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