Efficient micropropagation protocol for highbush blueberry (Vaccinium corymbosum L.) cv. 'Elliot'

被引:0
作者
Vescan, Liviu Adrian [1 ]
Pamfil, Doru [1 ]
Clapa, Doina [2 ]
Fira, Alexandru [2 ]
Sisea, Cristian Radu [1 ]
Pop, Iulia Francesca [1 ]
Petricele, Ioana Virginia [1 ]
Ciuzan, Oana [1 ]
Pop, Rodica [1 ]
机构
[1] Univ Agr Sci & Vet Med Cluj Napoca, Cluj Napoca 400372, Romania
[2] Fruit Res Stn Cluj, Cluj Napoca 400457, Romania
来源
ROMANIAN BIOTECHNOLOGICAL LETTERS | 2012年 / 17卷 / 01期
关键词
micropropagation; highbush blueberry; cv. 'Elliot'; plant growth regulators; IN-VITRO; CULTIVARS; CULTURES;
D O I
暂无
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Blueberries have a high nutritional value and strong antioxidant properties, qualities which account for their increasing request on the food and pharmaceutical markets. Cultivar 'Elliot' has a balanced content of active substances. It is also a late ripening cultivar suitable for long Transylvanian winters, which makes it a good candidate for large scale cultivation in this region. Because traditional propagation methods of highbush blueberry have several drawbacks, studies for creating an efficient micropropagation protocol were initiated. Dormant buds were used as initial explants for shoot regeneration while axillary and adventitious microshoots were further used for the proliferation stage. The influence of several plant growth regulators (2-iP, Zeatin, BAP) in relation with the explant positioning into media was investigated in order to ensure the highest proliferation rate. WPM supplemented with zeatin 2 mg l(-1) and 2-iP 5 mg l(-1) along with horizontal microshoot positioning gave the best proliferation rate, with an average of 17.9 shoots/explant. In vitro obtained leaves were tested for their regeneration capacity. The use of 2-iP 10 mg l(-1) produced up to 90% regeneration rate. The best variant consisted in abaxial leaf positioning which resulted in 50% direct regeneration rate and an average of 6.5 shoots/initial explant. The maximum proliferation rate was recorded for adaxial positioning (26 shoots/initial explant). Direct ex vitro acclimatization and rooting in hydroculture were also tested with very good results.
引用
收藏
页码:6893 / 6902
页数:10
相关论文
共 50 条
  • [41] The High-Bush Blueberry (Vaccinium corymbosum L.) Breeding Programme in Poland
    Pluta, S.
    Zurawicz, E.
    X INTERNATIONAL SYMPOSIUM ON VACCINIUM AND OTHER SUPERFRUITS, 2014, 1017 : 177 - 180
  • [42] Comparative study of volatile composition of major Northern Highbush blueberry (Vaccinium corymbosum) varieties
    Qian, Yan Ping L.
    Zhou, Qin
    Magana, Armando Alcazar
    Qian, Michael C.
    JOURNAL OF FOOD COMPOSITION AND ANALYSIS, 2022, 110
  • [43] The use of zeatin as growth regulator for the micrpropagation of some highbush blueberry (Vaccinium corymbosum) cultivars
    Clapa, Doina
    Fira, Al.
    BULLETIN OF THE UNIVERSITY OF AGRICULTURAL SCIENCES AND VETERINARY MEDICINE, VOL 63, 2006: HORTICULTURE, 2006, 63 : 400 - 400
  • [44] Micropropagation of the Morus nigra L. (Black Mulberry) cv. 'Eksi Kara'
    Ozelci, Duygu
    Yigit, Emel
    KSU TARIM VE DOGA DERGISI-KSU JOURNAL OF AGRICULTURE AND NATURE, 2022, 25 (01): : 49 - 56
  • [45] Mathematical modeling of the indole-3-butyric acid applications on rooting of northern highbush blueberry (Vaccinium corymbosum L.) softwood-cuttings
    Huseyin Celik
    Mehmet Serhat Odabas
    Acta Physiologiae Plantarum, 2009, 31 : 295 - 299
  • [46] ANTIOXIDANT COMPOUNDS IN SKIN AND PULP OF FRUITS CHANGE AMONG GENOTYPES AND MATURITY STAGES IN HIGHBUSH BLUEBERRY (Vaccinium corymbosum L.) GROWN IN SOUTHERN CHILE
    Ribera, A. E.
    Reyes-Diaz, M.
    Alberdi, M.
    Zuniga, G. E.
    Mora, M. L.
    JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION, 2010, 10 (04) : 509 - 536
  • [47] Optimization of an improved, efficient and rapid in vitro micropropagation protocol for Petunia hybrida Vilm. Cv. "Bravo"
    Farooq, Iqra
    Qadri, Z. A.
    Rather, Z. A.
    Nazki, Imtiyaz T.
    Banday, Neelofar
    Rafiq, Sadaf
    Masoodi, Khalid Z.
    Noureldeen, Ahmed
    Mansoor, Sheikh
    SAUDI JOURNAL OF BIOLOGICAL SCIENCES, 2021, 28 (07) : 3701 - 3709
  • [48] Mathematical modeling of the indole-3-butyric acid applications on rooting of northern highbush blueberry (Vaccinium corymbosum L.) softwood-cuttings
    Celik, Huseyin
    Odabas, Mehmet Serhat
    ACTA PHYSIOLOGIAE PLANTARUM, 2009, 31 (02) : 295 - 299
  • [49] Influence of Winter Temperatures on the Yielding Ability of Highbush Blueberries (Vaccinium corymbosum L.) Cultivars in Belarus
    Pavlovski, Nikolai
    INTERNATIONAL JOURNAL OF FRUIT SCIENCE, 2010, 10 (02) : 157 - 165
  • [50] ANALYSIS OF GENETIC STABILITY IN THE ex vitro ROOTED MICROCUTTINGS OF BLUEBERRY (Vaccinium corymbosum L.)
    Nowakowska, Karolina
    Pacholczak, Andrzej
    ACTA SCIENTIARUM POLONORUM-HORTORUM CULTUS, 2017, 16 (05): : 19 - 27