Expanding the genetic code of the human hematopoietic system

被引：19

作者：

Shao, Sida ^{[1
,2
]}

Koh, Minseob ^{[1
,2
]}

Schultz, Peter G. ^{[1
,2
]}

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2020年 / 117卷 / 16期

关键词：

genetic code expansion; Epstein-Barr virus; hematopoietic stem cell; hematopoietic reconstruction; STEM-CELLS; EXPANSION; DELIVERY; PROGRESS; BIOLOGY; MOUSE;

D O I：

10.1073/pnas.1914408117

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The genetic incorporation of noncanonical amino acids (ncAAs) into proteins has been realized in bacteria, yeast, and mammalian cells, and recently, in multicellular organisms including plants and animals. However, the addition of new building blocks to the genetic code of tissues from human origin has not yet been achieved. To this end, we report a self-replicating Epstein-Barr virus-based episomal vector for the long-term encoding of ncAAs in human hematopoietic stem cells and reconstitution of this genetically engineered hematopoietic system in mice.

引用

页码：8845 / 8849

页数：5

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