Isolation of human mesenchymal stem cells: bone marrow versus umbilical cord blood

被引:3
作者
Mareschi, K
Biasin, E
Piacibello, W
Aglietta, M
Madon, E
Fagioli, F
机构
[1] Univ Turin, Dept Pediat, I-10126 Turin, Italy
[2] Univ Turin, Dept Biomed Sci & Human Oncol, IRCCS Candiolo, I-10126 Turin, Italy
关键词
mesenchymal stem cell; bone marrow; cord blood;
D O I
暂无
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
We attempted the isolation and characterization of mesenchymal stem cells (MSCs) from bone marrow (BM) and umbilical cord blood (UBC) in a medium with 10% fetal bovine serum (FBS) and 10% horse serum. In the same conditions it was possible to isolate MSCs from bone marrow but not from UCB.
引用
收藏
页码:1099 / 1100
页数:2
相关论文
共 11 条
[1]   Mesenchymal progenitor cells in human umbilical cord blood [J].
Erices, A ;
Conget, P ;
Minguell, JJ .
BRITISH JOURNAL OF HAEMATOLOGY, 2000, 109 (01) :235-242
[2]  
FRIEDENSTEIN AJ, 1974, EXP HEMATOL, V2, P83
[3]   Characterization of the adherent cells developed in Dexter-type long-term cultures from human umbilical cord blood [J].
Gutiérrez-Rodríguez, M ;
Reyes-Maldonado, E ;
Mayani, H .
STEM CELLS, 2000, 18 (01) :46-52
[4]  
Majumdar MK, 1998, J CELL PHYSIOL, V176, P57, DOI 10.1002/(SICI)1097-4652(199807)176:1<57::AID-JCP7>3.0.CO
[5]  
2-7
[6]   Mesenchymal stem cells [J].
Minguell, JJ ;
Erices, A ;
Conget, P .
EXPERIMENTAL BIOLOGY AND MEDICINE, 2001, 226 (06) :507-520
[7]   Endothelial cell precursors are normal components of human umbilical cord blood [J].
Nieda, M ;
Nicol, A ;
DenningKendall, P ;
Sweetenham, J ;
Bradley, B ;
Hows, J .
BRITISH JOURNAL OF HAEMATOLOGY, 1997, 98 (03) :775-777
[8]   Multilineage potential of adult human mesenchymal stem cells [J].
Pittenger, MF ;
Mackay, AM ;
Beck, SC ;
Jaiswal, RK ;
Douglas, R ;
Mosca, JD ;
Moorman, MA ;
Simonetti, DW ;
Craig, S ;
Marshak, DR .
SCIENCE, 1999, 284 (5411) :143-147
[9]   Marrow stromal cells as steam cells for nonhematopoietic tissues [J].
Prockop, DJ .
SCIENCE, 1997, 276 (5309) :71-74
[10]   ESTABLISHMENT OF AN ADHERENT CELL FEEDER LAYER FROM HUMAN UMBILICAL-CORD BLOOD FOR SUPPORT OF LONG-TERM HEMATOPOIETIC PROGENITOR-CELL GROWTH [J].
YE, ZQ ;
BURKHOLDER, JK ;
QIU, P ;
SCHULTZ, JC ;
SHAHIDI, NT ;
YANG, NS .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (25) :12140-12144