Bacillus acidicola sp nov., a novel mesophilic, acidophilic species isolated from acidic Sphagnum peat bogs in Wisconsin

A mesophilic, acidophilic, spore-forming bacterium, strain 105-2(T), was isolated from an acidic Sphagnum peat bog in Wisconsin, USA. Strain 105-2(T) has 16S rRNA gene sequence similarity to Bacillus sporothermodurans DSM 10599(T) and Bacillus oleronius DSM 9356(T) of 97(.)4 and 97(.)8%, respectively. The primary lipoquinone is MK-7 and the major fatty acids are 15:0 iso, 15:0 anteiso and 17:0 anteiso. The predominant polar lipids; were found to be diphosphatidylglycerol, phosphatidylglycerol, phosphaticlylethanolamine, and a glycolipid. The DNA G + C content was found to be 43(.)2 mol%. The phenotypic, chemotaxonomic and molecular analyses identified strain 105-2(T) as a novel Bacillus species, for which the name Bacillus acidicola is proposed. The type strain is 105-2(T) (=DSM 14745(T) =ATCC BAA-366(T) =NRRL B-23453(T)).