Reversible addition-fragmentation chain transfer polymerization in DNA biosensing

被引：58

作者：

He, Peng ^{[1
]}

Zheng, Weiming ^{[1
]}

Tucker, Eric Z. ^{[1
]}

Gorman, Christopher B. ^{[1
]}

He, Lin ^{[1
]}

机构：

[1] N Carolina State Univ, Dept Chem, Raleigh, NC 27695 USA

来源：

ANALYTICAL CHEMISTRY | 2008年 / 80卷 / 10期

关键词：

D O I：

10.1021/ac702608k

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Reversible addition-fragmentation chain transfer polymerization is employed here to allow detector-free visualization of specific DNA sequences for which dynamic polymer growth is used in signal amplification. In particular, surface-initiated polymer growth was regulated by the immobilization of chain transfer agents on the An surface where DNA hybridization occurred. A linear polymer growth was observed as a function of the reaction time, characteristic of "living" polymer reactions. Significant improvement in assay sensitivity was realized in comparison to the previously reported polymerization-based sensing method by enhancing polymer growth rate and reducing background noises caused by nonspecific adsorption. Direct visualization of fewer than 2,000 copies of a short oligonucleotide sequence was demonstrated in a detector-free fashion.

引用

页码：3633 / 3639

页数：7

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