Conversion of just-continuous metallic films to large particulate substrates for metal-enhanced fluorescence

被引:44
作者
Aslan, Kadir
Malyn, Stuart N.
Zhang, Yongxia
Geddes, Chris D. [1 ]
机构
[1] Univ Maryland, Inst Biotechnol, Inst Fluorescence, Lab Adv Med Plasmon, Baltimore, MD 21201 USA
关键词
D O I
10.1063/1.2905319
中图分类号
O59 [应用物理学];
学科分类号
摘要
We report the effects of thermally annealing, non-, just-, and thick continuous silver films for their potential applications in metal-enhanced fluorescence, a near-field concept which can alter the free-space absorption and emissive properties of close-proximity fluorophores (excited states). We have chosen to anneal a noncontinuous particulate film 5 nm thick and two thicker continuous films, 15 and 25 nm thick, respectively. Our results show that the annealing of the 25 nm film has little effect on close-proximity fluorescence when coated with a monolayer of fluorophore-labeled protein. However, the 15 nm continuous film cracks upon annealing, producing large nanoparticles which are ideal for enhancing the fluorescence of close-proximity fluorophores that are indeed difficult to prepare by other wet-chemical deposition processes. The annealing of 5 nm noncontinuous particulate films (a control sample) has little influence on metal-enhanced fluorescence, as expected. (C) 2008 American Institute of Physics.
引用
收藏
页数:7
相关论文
共 36 条
[1]   Metal-enhanced fluorescence: an emerging tool in biotechnology [J].
Aslan, K ;
Gryczynski, I ;
Malicka, J ;
Matveeva, E ;
Lakowicz, JR ;
Geddes, CD .
CURRENT OPINION IN BIOTECHNOLOGY, 2005, 16 (01) :55-62
[2]   Metal-enhanced fluorescence-based RNA sensing [J].
Aslan, K ;
Huang, J ;
Wilson, GM ;
Geddes, CD .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (13) :4206-4207
[3]   Annealed silver-island films for applications in metal-enhanced fluorescence: Interpretation in terms of radiating plasmons [J].
Aslan, K ;
Leonenko, Z ;
Lakowicz, JR ;
Geddes, CD .
JOURNAL OF FLUORESCENCE, 2005, 15 (05) :643-654
[4]   Angular-ratiometric plasmon-resonance based light scattering for bioaffinity sensing [J].
Aslan, K ;
Holley, P ;
Davies, L ;
Lakowicz, JR ;
Geddes, CD .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (34) :12115-12121
[5]  
Aslan K, 2007, BIOPHYS J, p371A
[6]   Angular-dependent metal-enhanced fluorescence from silver colloid-deposited films: opportunity for angular-ratiometric surface assays [J].
Aslan, Kadir ;
Malyn, Stuart N. ;
Geddes, Chris D. .
ANALYST, 2007, 132 (11) :1112-1121
[7]   Metal-enhanced fluorescence from silver nanoparticle-deposited polycarbonate substrates [J].
Aslan, Kadir ;
Holley, Patrick ;
Geddes, Chris D. .
JOURNAL OF MATERIALS CHEMISTRY, 2006, 16 (27) :2846-2852
[8]   Microwave-accelerated and metal-enhanced fluorescence myoglobin detection on silvered surfaces: Potential application to myocardial infarction diagnosis [J].
Aslan, Kadir ;
Geddes, Chris D. .
PLASMONICS, 2006, 1 (01) :53-59
[9]   Metal-enhanced fluorescence from gold surfaces: Angular dependent emission [J].
Aslan, Kadir ;
Malyn, Stuart N. ;
Geddes, Chris D. .
JOURNAL OF FLUORESCENCE, 2007, 17 (01) :7-13
[10]   Metal-enhanced chemiluminescence: Radiating plasmons generated from chemically induced electronic excited states [J].
Chowdhury, MH ;
Aslan, K ;
Malyn, SN ;
Lakowicz, JR ;
Geddes, CD .
APPLIED PHYSICS LETTERS, 2006, 88 (17)