Superoxide generation from constitutive nitric oxide synthase in astrocytes in vitro regulates extracellular nitric oxide availability

被引：31
作者：
Tolias, CM [1 ]
McNeil, CJ
Kazlauskaite, J
Hillhouse, EW
机构：
[1] Univ Warwick, Dept Biol Sci, Sir Quinton Hazell Mol Med Res Ctr, Coventry CV4 7AL, W Midlands, England
[2] Univ Newcastle Upon Tyne, Sch Med, Dept Clin Biochem, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England
来源：
FREE RADICAL BIOLOGY AND MEDICINE | 1999年 / 26卷 / 1-2期
基金：
英国惠康基金;
关键词：
constitutive nitric oxide synthase; superoxide; nitric oxide; primary cortical cell cultures; astrocytes; electrochemical sensors; free radical;
D O I：
10.1016/S0891-5849(98)00146-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Oxygen-derived free radicals play an important role in the physiology and pathophysiology of brain cell function. Because of their labile nature, however, it has been difficult to investigate their actions directly. This problem has been addressed, in primary rat brain cell cultures, in this study by utilization of two novel electrochemical sensors. It has been demonstrated that extracellular superoxide originates from the astrocytic subpopulation in a calcium/calmodulin dependent manner and responds to constitutive nitric oxide synthase inhibition. The results indicate a novel function for the astrocytic constitutive nitric oxide synthase in regulating extracellular superoxide release and, therefore, controlling neuronal nitric oxide availability. (C) 1998 Elsevier Science Inc.
引用
收藏
页码：99 / 106
页数：8
相关论文
共 32 条
1234