Correlation between mast cell density and myocardial fibrosis in congestive heart failure patients

被引:43
作者
Batlle, M.
Perez-Villa, F.
Lazaro, A.
Garcia-Pras, E.
Ramirez, J.
Ortiz, J.
Orus, J.
Roque, M.
Heras, M.
Roig, E.
机构
[1] Hosp Clin Barcelona, Inst Clin Torax, E-08036 Barcelona, Spain
[2] Hosp Clin Barcelona, Inst Invest Biomed August Pi & Sunyer IDIBAPS, E-08036 Barcelona, Spain
[3] Hosp Clin Barcelona, Dept Anat Patol, E-08036 Barcelona, Spain
关键词
D O I
10.1016/j.transproceed.2007.06.047
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Besides the well-established role of mast cells in allergic reactions as an important source of vasoactive and proinflammatory products, they have been related to tissue fibrosis and remodelling processes. In a heart failure (HF) animal model, mast cells were shown to synthesize transforming growth factor beta 1. and basic fibroblast growth factor in myocardial tissue and were localized to an area with fibrosis. Our objective was to quantify mast cell density in left ventricles from patients with congestive heart failure who were candidates for transplantation and to analyze whether they showed a correlation with the fibrosis level of the same area. Methods. We obtained myocardial biopsies from 20 patients with end-stage HF secondary to idiopathic dilated cardiomyopathy (iDCM) undergoing heart transplantation and 15 controls (donors without cardiopathy). Mast cells were detected by immunohistochemistry with a human mast cell chymase antibody and fibrosis levels measured with picrosirius red staining of collagen fibrils with later quantification by morphometry. Results. The patients mean age was 51 3 years. Fibrosis levels in the myocardial sections from patients with congestive HF was three-fold higher than those in control myocardium (12.41 +/- 1.7% vs 3.98 +/- 0.63%, P <.001). Mast cell density correlated with the collagen fraction and could be fitted to a linear regression curve: collagen fraction = 0.78 + 0.05 mast cell density (n = 33, P <.005, R-2 = 0.28). Conclusion. The elevated collagen fraction present in failing hearts may be the cause of increased stiffness and loss of elasticity that is detected in patients with end-stage HE Due to the mast cells capacity to synthesize vasoactive and fibrogenic products and the correlation between their density and fibrosis levels, they probably play a role in the ventricular remodelling in HF.
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页码:2347 / 2349
页数:3
相关论文
共 8 条
[1]   Increased expression of the renin-angiotensin system and mast cell density but not of angiotensin-converting enzyme II in late stages of human heart failure [J].
Batlle, Montserrat ;
Roig, Eulalia ;
Perez-Villa, Felix ;
Lario, Sergio ;
Cejudo-Martin, Pilar ;
Garcia-Pras, Ester ;
Ortiz, Jose ;
Roque, Merce ;
Orus, Josefina ;
Rigol, Montserrat ;
Heras, Magdalena ;
Ramirez, Jose ;
Jimenez, Wladimiro .
JOURNAL OF HEART AND LUNG TRANSPLANTATION, 2006, 25 (09) :1117-1125
[2]   Cardiac collagen remodeling in the cardiomyopathic Syrian hamster and the effect of Losartan [J].
Dixon, IMC ;
Ju, HS ;
Reid, NL ;
ScammellLaFleur, T ;
Werner, JP ;
Jasmin, G .
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 1997, 29 (07) :1837-1850
[3]   The inflammatory response in myocardial infarction [J].
Frangogiannis, NG ;
Smith, CW ;
Entman, ML .
CARDIOVASCULAR RESEARCH, 2002, 53 (01) :31-47
[4]  
GALLI SJ, 1993, NEW ENGL J MED, V328, P257
[5]  
Gruber BL, 1997, J IMMUNOL, V158, P2310
[6]   Differential effects of angiotensin II on cardiac cell proliferation and intramyocardial perivascular fibrosis in vivo [J].
McEwan, PE ;
Gray, GA ;
Sherry, L ;
Webb, DJ ;
Kenyon, CJ .
CIRCULATION, 1998, 98 (24) :2765-2773
[7]   A role for cardiac mast cells in the pathogenesis of hypertensive heart disease [J].
Shiota, N ;
Rysä, J ;
Kovanen, PT ;
Ruskoaho, H ;
Kokkonen, JO ;
Lindstedt, KA .
JOURNAL OF HYPERTENSION, 2003, 21 (10) :1935-1944
[8]   Development, significance, and heterogeneity of mast cells with particular regard to the mast cell-specific proteases chymase and tryptase [J].
Welle, M .
JOURNAL OF LEUKOCYTE BIOLOGY, 1997, 61 (03) :233-245