Determination of sulfonamides in serum by on-line solid-phase extraction coupled to liquid chromatography with photoinduced fluorescence detection

被引：21

作者：

Arroyo-Manzanares, Natalia ^{[1
]}

Lara, Francisco J. ^{[1
]}

Airado-Rodriguez, Diego ^{[1
]}

Gamiz-Gracia, Laura ^{[1
]}

Garcia-Campana, Ana M. ^{[1
]}

机构：

[1] Univ Granada, Dept Analyt Chem, Fac Sci, E-18071 Granada, Spain

来源：

TALANTA | 2015年 / 138卷

关键词：

On-line solid-phase extraction; Sulfonamides; Liquid chromatography; Photoinduced fluorescence; Serum; PHOTOCHEMICALLY INDUCED FLUORESCENCE; TANDEM MASS-SPECTROMETRY; PHARMACEUTICAL-PREPARATIONS; SENSITIVE DETERMINATION; QUANTITATIVE-ANALYSIS; HUMAN URINE; MILK; WATER; LC; ANTIBIOTICS;

D O I：

10.1016/j.talanta.2015.03.012

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An analytical method based on on-line solid-phase extraction coupled to liquid chromatography with photoinduced fluorescence detection has been developed to determine sulfonamides in serum. A home-made setup was used to percolate 3 mL of sample through a solid-phase extraction column. Analytes were retained onto the sorbent by an anion exchange mechanism which ensures an optimum compatibility with the subsequent chromatographic separation using a C-18 column and an on-line photoreactor in order to derivatize sulfonamides, which do not present native fluorescence. The method allowed the determination of 7 sulfonamides in serum samples previously deproteinized in less than 18 mm and with limits of detection ranging between 1.8 and 3.6 mg/L. Relative recoveries between 91.5% and 102.1% were obtained with satisfactory precision since relative standard deviations were always below 10.5%. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：258 / 262

页数：5

共 55 条

[1] QUANTITATIVE-ANALYSIS OF SULFADIAZINE USING PHOTOCHEMICALLY INDUCED FLUORESCENCE DETECTION IN BULK SOLUTION AND IN A FLOW-INJECTION SYSTEM [J].