Histological analysis and identification of spermatogenesis-related genes in 2-, 6-, and 12-month-old sheep testes

被引：20

作者：

Bai, Man ^{[1
]}

Sun, Limin ^{[1
]}

Zhao, Jia ^{[1
]}

Xiang, Lujie ^{[1
]}

Cheng, Xiaoyin ^{[1
]}

Li, Jiarong ^{[1
]}

Jia, Chao ^{[1
]}

Jiang, Huaizhi ^{[1
]}

机构：

[1] Jilin Agr Univ, Coll Anim Sci & Technol, Changchun 130118, Jilin, Peoples R China

来源：

SCIENCE OF NATURE | 2017年 / 104卷 / 9-10期

关键词：

Sheep; Testis development; Spermatogenesis; Histological analysis; Transcriptome analysis; NUCLEOTIDE EXCISION-REPAIR; TRANSCRIPTOME ANALYSIS; MALE-FERTILITY; PROTEIN; EXPRESSION; ADIPONECTIN; RECEPTORS; OVULATION; ADIPOR2; CELLS;

D O I：

10.1007/s00114-017-1505-1

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Testis development and spermatogenesis are vital factors that influence male animal fertility. In order to identify spermatogenesis-related genes and further provide a theory basis for finding biomarkers related to male sheep fertility, 2-, 6-, and 12-month-old Small Tail Han Sheep testes were selected to investigate the dynamic changes of sheep testis development. Hematoxylin-eosin routine staining and RNA-Seq technique were used to perform histological and transcriptome analysis for these testes. The results showed that 630, 102, and 322 differentially expressed genes (DEGs) were identified in 2- vs 6-month-old, 6- vs 12-month-old, and 2- vs 12-month-old testes, respectively. GO and KEGG analysis showed the following: DEGs in 2- vs 6-month-old testes were mainly related to the GO terms of sexual maturation and the pathways of multiple metabolism and biosynthesis; in 6 -vs 12-month-old testes, most of the GO terms that DEGs involved in were related to metabolism and translation processes; the most significantly enriched pathway is the ribosome pathway. The union of DEGs in 2- vs 6-month-old, 6- vs 12month-old, and 2- vs 12-month-old testes was categorized into eight profiles by series cluster. Subsequently, the eight profiles were classified into four model profiles and four co-expression networks were constructed based on the DEGs in these model profiles. Finally, 29 key regulatory genes related to spermatogenesis were identified in the four co-expression networks. The expression of 13 DEGs (CA3, APOH, MYOC, CATSPER4, SYT6, SERPINA10, DAZL, ADIPOR2, RAB13, CEP41, SPAG4, ODF1, and FRG1) was validated by RT-PCR.

引用

页数：13

共 44 条

[1] Role of selenium in male reproduction-A review [J].

Ahsan, U. ;

Kamran, Z. ;

Raza, I. ;

Ahmad, S. ;

Babar, W. ;

Riaz, M. H. ;

Iqbal, Z. .

ANIMAL REPRODUCTION SCIENCE, 2014, 146 (1-2) :55-62

[2] Network biology:: Understanding the cell's functional organization [J].

Barabási, AL ;

Oltvai, ZN .

NATURE REVIEWS GENETICS, 2004, 5 (02) :101-U15

[3] Transcriptomic analysis of hepatic responses to testosterone deficiency in miniature pigs fed a high-cholesterol diet [J].

Cai, Zhaowei ;

Jiang, Xiaoling ;

Pan, Yongming ;

Chen, Liang ;

Zhang, Lifan ;

Zhu, Keyan ;

Cai, Yueqin ;

Ling, Yun ;

Chen, Fangming ;

Xu, Xiaoping ;

Chen, Minli .

BMC GENOMICS, 2015, 16

[4] Uncommon nucleotide excision repair phenotypes revealed by targeted high-throughput sequencing [J].

Calmels, Nadege ;

Greff, Geraldine ;

Obringer, Cathy ;

Kempf, Nadine ;

Gasnier, Claire ;

Tarabeux, Julien ;

Miguet, Marguerite ;

Baujat, Genevieve ;

Bessis, Didier ;

Bretones, Patricia ;

Cavau, Anne ;

Digeon, Beatrice ;

Doco-Fenzy, Martine ;

Doray, Berenice ;

Feillet, Francois ;

Gardeazabal, Jesus ;

Gener, Blanca ;

Julia, Sophie ;

Llano-Rivas, Isabel ;

Mazur, Artur ;

Michot, Caroline ;

Renaldo-Robin, Florence ;

Rossi, Massimiliano ;

Sabouraud, Pascal ;

Keren, Boris ;

Depienne, Christel ;

Muller, Jean ;

Mandel, Jean-Louis ;

Laugel, Vincent .

ORPHANET JOURNAL OF RARE DISEASES, 2016, 11

[5] Effects of GnRH administration on ovulation and fertility in ewes subjected to estrous synchronization [J].

Cavalcanti, Amanda dos Santos ;

Brandao, Felipe Zandonadi ;

Garcia Nogueira, Luiz Altamiro ;

da Fonseca, Jeferson Ferreira .

REVISTA BRASILEIRA DE ZOOTECNIA-BRAZILIAN JOURNAL OF ANIMAL SCIENCE, 2012, 41 (06) :1412-1418

[6] Expression of adiponectin and its receptors (AdipoR1 and AdipoR2) in chicken ovary: Potential role in ovarian steroidogenesis [J].

Chabrolle, Christine ;

Tosca, Lucie ;

Crochet, Sabine ;

Tesseraud, Sophie ;

Dupont, Joealle .

DOMESTIC ANIMAL ENDOCRINOLOGY, 2007, 33 (04) :480-487

[7] Genes related to the very early stage of ConA-induced fulminant hepatitis: a gene-chip-based study in a mouse model [J].

Chen, Feng ;

Zhu, Hai-Hong ;

Zhou, Lin-Fu ;

Li, Jie ;

Zhao, Li-Ying ;

Wu, Shan-Shan ;

Wang, Jing ;

Liu, Wei ;

Chen, Zhi .

BMC GENOMICS, 2010, 11

[8] DAZL Limits Pluripotency, Differentiation, and Apoptosis in Developing Primordial Germ Cells [J].

Chen, Hsu-Hsin ;

Welling, Maaike ;

Bloch, Donald B. ;

Munoz, Javier ;

Mientjes, Edwin ;

Chen, Xinjie ;

Tramp, Cody ;

Wu, Jie ;

Yabuuchi, Akiko ;

Chou, Yu-Fen ;

Buecker, Christa ;

Krainer, Adrian ;

Willemsen, Rob ;

Heck, Albert J. ;

Geijsen, Niels .

STEM CELL REPORTS, 2014, 3 (05) :892-904

[9]

Chen Jing, 2009, Zhonghua Nan Ke Xue, V15, P891

[10] Uncovering the Complexity of Transcriptomes with RNA-Seq [J].

Costa, Valerio ;

Angelini, Claudia ;

De Feis, Italia ;

Ciccodicola, Alfredo .

JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY, 2010,

← 1 2 3 4 5 →