Conformation Research of Shorted Glucagon-like Peptide-1

Shorted glucagon-like peptide-1 (sGLP-1) not only stimulate proliferation of pancreatic beta cells and decrease blood glucose, but also has a longer half-life. This make sGLP-1 has become a potential new drug in the future. In order to elucidate the function of sGLP-1, this study investigates the conformation of sGLP-1 in different conditions by circular dichroism spectrum and fluorescence spectrum. The conformations of sGLP-1 in phosphate buffer are mostly unordered structure and very low helix, and are mostly a-helix in phosphate buffer containing SDS or TFE. Temperature and pH values of solutions have great influences on the conformation of sGLP-1. sGLP-1 preserve the critical residues which are responsible for binding and activating receptor, Induced by receptor., sGLP-1 can form the correct conformation which can bind and activate receptor in vivo, then realize its function. The results show that appropriately truncate C-terminal segment of GLP-1, may obtain long-term and high efficiency GLP-1 analogues.