Effects of basic fibroblast growth factor on proliferation and phenotype expression of chondrocytes embedded in collagen gel

被引：36

作者：

Matsusaki, M ^{[1
]}

Ochi, M ^{[1
]}

Uchio, Y ^{[1
]}

Shu, N ^{[1
]}

Kurioka, H ^{[1
]}

Kawasaki, K ^{[1
]}

Adachi, N ^{[1
]}

机构：

[1] Shimane Med Univ, Dept Orthopaed, Izumo, Shimane 6938501, Japan

来源：

GENERAL PHARMACOLOGY-THE VASCULAR SYSTEM | 1998年 / 31卷 / 05期

关键词：

basic fibroblast growth factor; chondrocyte; collagen gel;

D O I：

10.1016/S0306-3623(98)00105-0

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

1. Whether basic fibroblast growth factor (bFGF) can stimulate proliferation and synthesis of chondroitin sulfate of chondrocytes in gel culture while maintaining the phenotype was studied. 2. At 3 weeks in culture, the cell number in 1.0 ng/ml (18.5 +/- 2.1 x 10(5)) and 10.0 ng/ml of the bFGF group (15.3 +/- 1.9 x 10(5)) was significantly larger than that in 0 ng/ml (12.3 +/- 2.1 x 10(5)), 0.1 ng/ml (11.7 +/- 2.2 x 10(5)) and 100.0 ng/ml of the bFGF group (9.8 +/- 2.3 x 10(5)). 3. All doses of bFGF used in this study suppressed synthesis of chondroitin 6-sulfate. 4. Chondrocyte phenotype in gel culture was maintained for 4 weeks even with stimulation of bFGF. (C) 1998 Elsevier Science Inc.

引用

页码：759 / 764

页数：6

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