Effects of basic fibroblast growth factor on proliferation and phenotype expression of chondrocytes embedded in collagen gel

被引：36

作者：

Matsusaki, M ^{[1
]}

Ochi, M ^{[1
]}

Uchio, Y ^{[1
]}

Shu, N ^{[1
]}

Kurioka, H ^{[1
]}

Kawasaki, K ^{[1
]}

Adachi, N ^{[1
]}

机构：

[1] Shimane Med Univ, Dept Orthopaed, Izumo, Shimane 6938501, Japan

来源：

GENERAL PHARMACOLOGY-THE VASCULAR SYSTEM | 1998年 / 31卷 / 05期

关键词：

basic fibroblast growth factor; chondrocyte; collagen gel;

D O I：

10.1016/S0306-3623(98)00105-0

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

1. Whether basic fibroblast growth factor (bFGF) can stimulate proliferation and synthesis of chondroitin sulfate of chondrocytes in gel culture while maintaining the phenotype was studied. 2. At 3 weeks in culture, the cell number in 1.0 ng/ml (18.5 +/- 2.1 x 10(5)) and 10.0 ng/ml of the bFGF group (15.3 +/- 1.9 x 10(5)) was significantly larger than that in 0 ng/ml (12.3 +/- 2.1 x 10(5)), 0.1 ng/ml (11.7 +/- 2.2 x 10(5)) and 100.0 ng/ml of the bFGF group (9.8 +/- 2.3 x 10(5)). 3. All doses of bFGF used in this study suppressed synthesis of chondroitin 6-sulfate. 4. Chondrocyte phenotype in gel culture was maintained for 4 weeks even with stimulation of bFGF. (C) 1998 Elsevier Science Inc.

引用

页码：759 / 764

页数：6

共 30 条

[1] REPAIR OF ARTICULAR SURFACES BY ALLOGRAFTS OF ARTICULAR AND GROWTH-PLATE CARTILAGE [J].

ASTON, JE ;

BENTLEY, G .

JOURNAL OF BONE AND JOINT SURGERY-BRITISH VOLUME, 1986, 68 (01) :29-35

[2] HOMOTRANSPLANTATION OF ISOLATED EPIPHYSEAL AND ARTICULAR CARTILAGE CHONDROCYTES INTO JOINT SURFACES OF RABBITS [J].

BENTLEY, G ;

GREER, RB .

NATURE, 1971, 230 (5293) :385-&

[3]

Brittberg M, 1996, CLIN ORTHOP RELAT R, P270

[4] TREATMENT OF DEEP CARTILAGE DEFECTS IN THE KNEE WITH AUTOLOGOUS CHONDROCYTE TRANSPLANTATION [J].

BRITTBERG, M ;

LINDAHL, A ;

NILSSON, A ;

OHLSSON, C ;

ISAKSSON, O ;

PETERSON, L .

NEW ENGLAND JOURNAL OF MEDICINE, 1994, 331 (14) :889-895

[5]

CHESTERMAN P J, 1968, Journal of Bone and Joint Surgery British Volume, V50B, P184

[6] Effect of basic fibroblast growth factor on cartilage regeneration in chondrocyte-seeded collagen sponge scaffold [J].

Fujisato, T ;

Sajiki, T ;

Liu, Q ;

Ikada, Y .

BIOMATERIALS, 1996, 17 (02) :155-162

[7]

GREEN WT, 1977, CLIN ORTHOP RELAT R, P237

[8] REGULATION OF COLONY FORMATION OF DIFFERENTIATED CHONDROCYTES IN SOFT AGAR BY TRANSFORMING GROWTH FACTOR-BETA [J].

IWAMOTO, M ;

SATO, K ;

NAKASHIMA, K ;

FUCHIHATA, H ;

SUZUKI, F ;

KATO, Y .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 159 (03) :1006-1011

[9] FIBROBLAST GROWTH-FACTOR STIMULATES COLONY FORMATION OF DIFFERENTIATED CHONDROCYTES IN SOFT AGAR [J].

KATO, Y ;

IWAMOTO, M ;

KOIKE, T .

JOURNAL OF CELLULAR PHYSIOLOGY, 1987, 133 (03) :491-498

[10]

KATO Y, 1990, J BIOL CHEM, V265, P5903

← 1 2 3 →