Long-term survival in non-human primates of stem cell-derived, MHC-unmatched corneal epithelial cell sheets

被引:11
作者
Yoshinaga, Yu [1 ]
Soma, Takeshi [1 ]
Azuma, Shohei [1 ]
Maruyama, Kazuichi [2 ]
Hashikawa, Yoshiko [1 ,3 ,4 ]
Katayama, Tomohiko [1 ]
Sasamoto, Yuzuru [5 ]
Takayanagi, Hiroshi [1 ,9 ]
Hosen, Naoki [6 ]
Shiina, Takashi [7 ]
Ogasawara, Kazumasa [8 ]
Hayashi, Ryuhei [1 ,9 ,10 ]
Nishida, Kohji [1 ,10 ]
机构
[1] Osaka Univ, Grad Sch Med, Dept Ophthalmol, Suita, Osaka 5650871, Japan
[2] Osaka Univ, Grad Sch Med, Dept Innovat Visual Sci, Suita, Osaka 5650871, Japan
[3] Tohoku Univ, Grad Sch Med, United Ctr Adv Res, Div Clin Cell Therapy, Sendai, Miyagi 9808575, Japan
[4] Tohoku Univ, Grad Sch Med, United Ctr Translat Med, Div Clin Cell Therapy, Sendai, Miyagi 9808575, Japan
[5] Brigham & Womens Hosp, Dept Med, Boston, MA 02115 USA
[6] Osaka Univ, Grad Sch Med, Dept Hematol & Oncol, Suita, Osaka 5650871, Japan
[7] Tokai Univ, Sch Med, Dept Mol Life Sci, Isehara, Kanagawa 2591193, Japan
[8] Shiga Univ Med Sci, Dept Pathol, Otsu, Shiga 5202192, Japan
[9] Osaka Univ, Grad Sch Med, Dept Stem Cells & Appl Med, Suita, Osaka 5650871, Japan
[10] Osaka Univ, Inst Open & Transdisciplinary Res Initiat, Integrated Frontier Res Med Sci Div, Suita, Osaka 5650871, Japan
来源
STEM CELL REPORTS | 2022年 / 17卷 / 07期
关键词
INDUCED PLURIPOTENT; KERATOLIMBAL ALLOGRAFT; EX-VIVO; TRANSPLANTATION; IMMUNOGENICITY; SUPPRESSION;
D O I
10.1016/j.stemcr.2022.05.018
中图分类号
Q813 [细胞工程];
学科分类号
摘要
When corneal epithelial stem cells residing in the corneal limbus become dysfunctional, called a limbal stem cell deficiency (LSCD), corneal transparency is decreased, causing severe vision loss. Transplantation of corneal epithelial cell sheets (CEPS) derived from stem cells, including induced pluripotent stem cells, is a promising treatment for LSCD. However, the potential effect of human leukocyte antigen (HLA) concordance on CEPS transplantation has not been addressed. Here, we show that there is no difference in the immune response to CEPS between HLA-matched and -unmatched peripheral blood mononuclear cells in mixed lymphocyte reactions. CEPS transplantation in cynomolgus monkeys revealed that the immune response to major histocompatibility-unmatched CEPS was not strong and could be controlled by local steroid administration. Furthermore, programmed death ligand 1 was identified as an immunosuppressive molecule in CEPS under inflammatory conditions in vitro. Our results indicate that corneal epithelium has low immunogenicity and allogeneic CEPS transplantation requires mild immunosuppression.
引用
收藏
页码:1714 / 1729
页数:16
相关论文
共 43 条
  • [1] Negligible immunogenicity of terminally differentiated cells derived from induced pluripotent or embryonic stem cells
    Araki, Ryoko
    Uda, Masahiro
    Hoki, Yuko
    Sunayama, Misato
    Nakamura, Miki
    Ando, Shunsuke
    Sugiura, Mayumi
    Ideno, Hisashi
    Shimada, Akemi
    Nifuji, Akira
    Abe, Masumi
    [J]. NATURE, 2013, 494 (7435) : 100 - 104
  • [2] Brostjan C, 1997, J IMMUNOL, V158, P3836
  • [3] Clinical and pathologic findings in human keratolimbal allograft rejection
    Daya, SM
    Bell, RWD
    Habib, NE
    Powell-Richards, A
    Dua, HS
    [J]. CORNEA, 2000, 19 (04) : 443 - 450
  • [4] Immunosuppressive strategies in transplantation
    Denton, MD
    Magee, CC
    Sayegh, MH
    [J]. LANCET, 1999, 353 (9158) : 1083 - 1091
  • [5] PD-1/PD-L1 immune checkpoint: Potential target for cancer therapy
    Dermani, Fatemeh K.
    Samadi, Pouria
    Rahmani, Golebagh
    Kohlan, Alisa K.
    Najafi, Rezvan
    [J]. JOURNAL OF CELLULAR PHYSIOLOGY, 2019, 234 (02) : 1313 - 1325
  • [6] Human embryonic stem cells and their differentiated derivatives are less susceptible to immune rejection than adult cells
    Drukker, Micha
    Katchman, Helena
    Katz, Gil
    Friedman, Smadar Even-Tov
    Shezen, Elias
    Hornstein, Eran
    Mandelboim, Ofer
    Reisner, Yair
    Benvenisty, Nissim
    [J]. STEM CELLS, 2006, 24 (02) : 221 - 229
  • [7] PD-L1 regulates the development, maintenance, and function of induced regulatory T cells
    Francisco, Loise M.
    Salinas, Victor H.
    Brown, Keturah E.
    Vanguri, Vijay K.
    Freeman, Gordon J.
    Kuchroo, Vijay K.
    Sharpe, Arlene H.
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 2009, 206 (13) : 3015 - 3029
  • [8] Identification and characterization of a WT1 (Wilms tumor gene) protein-derived HLA-DRB1*0405-restricted 16-mer helper peptide that promotes the induction and activation of WT1-specific cytotoxic T lymphocytes
    Fujiki, Fumihiro
    Oka, Yoshihiro
    Tsuboi, Akihiro
    Kawakami, Manabu
    Kawakatsu, Mai
    Nakajima, Hiroko
    Elisseeva, Olga A.
    Harada, Yukie
    Ito, Ken
    Li, Zheyu
    Tatsumi, Naoya
    Sakaguchi, Nao
    Fujioka, Tatsuya
    Masuda, Tomoki
    Yasukawa, Masaki
    Udaka, Keiko
    Kawase, Ichiro
    Oji, Yusuke
    Sugiyama, Haruo
    [J]. JOURNAL OF IMMUNOTHERAPY, 2007, 30 (03) : 282 - 293
  • [9] Global Survey of Corneal Transplantation and Eye Banking
    Gain, Philippe
    Jullienne, Remy
    He, Zhiguo
    Aldossary, Mansour
    Acquart, Sophie
    Cognasse, Fabrice
    Thuret, Gilles
    [J]. JAMA OPHTHALMOLOGY, 2016, 134 (02) : 167 - 173
  • [10] The Role of Human Leukocyte Antigen Matching in the Development of Multiethnic "Haplobank" of Induced Pluripotent Stem Cell Lines
    Gourraud, Pierre-Antoine
    Gilson, Leena
    Girard, Mathilde
    Peschanski, Marc
    [J]. STEM CELLS, 2012, 30 (02) : 180 - 186