Selective biosensing of Staphylococcus aureus using chitosan quantum dots

被引：44

作者：

Abdelhamid, Hani Nasser ^{[1
,2
,3
]}

Wu, Hui-Fen ^{[1
,2
,4
,5
,6
,7
]}

机构：

[1] Natl Sun Yat Sen Univ, Dept Chem, Kaohsiung 804, Taiwan

[2] Kaohsiung Med Univ, Sch Pharm, Coll Pharm, Kaohsiung 800, Taiwan

[3] Assiut Univ, Dept Chem, Assiut 71515, Egypt

[4] Natl Sun Yat Sen Univ, Ctr Nanosci & Nanotechnol, Kaohsiung 804, Taiwan

[5] Natl Sun Yat Sen Univ, Doctoral Degree Program Marine Biotechnol, Kaohsiung 80424, Taiwan

[6] Acad Sinica, Kaohsiung 80424, Taiwan

[7] Natl Sun Yat Sen Univ, Inst Med Sci & Technol, Kaohsiung 804, Taiwan

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2018年 / 188卷

关键词：

Biosensing; Quantum dots; Pathogenic bacteria; Catalase; Fluorescence; Staphylococcus aureus; DESORPTION/IONIZATION MASS-SPECTROMETRY; BOVINE SERUM-ALBUMIN; PATHOGENIC BACTERIA; IN-VIVO; GRAPHENE OXIDE; LABEL-FREE; NANOPARTICLES; APTAMER; CDTE; CELLS;

D O I：

10.1016/j.saa.2017.06.047

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

Selective biosensing of Staphylococcus aureus (S. aureus) using chitosan modified quantum dots (CTS@CdS QDs) in the presence of hydrogen peroxide is reported. The method is based on the intrinsic positive catalase activity of S. aureus. CTS@CdS quantum dots provide high dispersion in aqueous media with high fluorescence emission. Staphylococcus aureus causes a selective quenching of the fluorescence emission of CTS@CdS QDs in the presence of H2O2 compared to other pathogens such as Escherichia coli and Pseudomonas aeruginosa. The intrinsic enzymatic character of S. aureus (catalase positive) offers selective and fast biosensing. The present method is highly selective for positive catalase species and requires no expensive reagents such as antibodies, aptamers or microbeads. It could be extended for other species that are positive catalase. (C) 2017 Published by Elsevier B.V.

引用

页码：50 / 56

页数：7

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