Methylene blue mediated photobiomodulation on human osteoblast cells

被引:35
作者
Ates, Gamze Bolukbasi [1 ]
Ak, Ayse [2 ]
Garipcan, Bora [1 ]
Gulsoy, Murat [1 ]
机构
[1] Bogazici Univ, Inst Biomed Engn, TR-34684 Istanbul, Turkey
[2] Erzincan Univ, Engn Fac, Biomed Engn, TR-24100 Erzincan, Turkey
关键词
Photobiomodulation (PBM); Photodynamic therapy (PDT); Osteoblast; Methylene blue; Low-level laser therapy (LLLT); 635; nm; LEVEL LASER THERAPY; PHOTODYNAMIC-THERAPY; IN-VITRO; STEM-CELLS; PROMOTES PROLIFERATION; IRRADIATION; DIFFERENTIATION; LIGHT; GROWTH; PHOTOCHEMOTHERAPY;
D O I
10.1007/s10103-017-2286-7
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Photobiomodulation (PBM) and photodynamic therapy (PDT) are two major methods, which use light in medicine and dentistry. PBM uses low-level laser light to induce cell proliferation and activity. In contrast, PDT use laser light combined with a photosensitizer (PS) to cause cell death. Due to similar, not fully understood mechanisms and biphasic response of light, unexpected and complex outcomes may be observed. In the present study, the effect of 635 nm laser light, with power density 50 mW/cm(2), at three different energy densities (0.5, 1, and 2 J/cm(2) which last 10, 20, and 40 s, respectively) mediated by methylene blue (MB) on the human osteoblast cell line (ATCC-CRL-11372, Rockville, MD, USA) was investigated. Cell viability (MTT assay and acridine orange/propidium iodide staining) and proliferation (Alamar Blue assay) were assessed at 24, 48, and 72 h post irradiation. Alkaline phosphatase (ALP) activity, mineralization (Alizarin Red staining) and gene expressions (RT-PCR analysis) were analyzed at 7th and 14th days after treatment. Five groups were formed as the control group (no MB, no irradiation), MB (only 0.05 mu M MB), MB + 0.5 J/cm(2), MB + 1 J/cm(2), and MB + 2 J/cm(2). Cell viability was decreased at 72 h (ANOVA; p < 0.05) for MB + 0.5 J/cm(2), MB + 1 J/cm(2), and MB + 2 J/cm(2) groups. Although proliferation does not seem to be effected by MB-mediated laser application, osteo-anabolic activity is altered. ALP activity was significantly increased at day 7 (ANOVA; p < 0.05) for MB-combined laser groups; on the other hand, mineralization was significantly decreased (ANOVA; p < 0.05) in all treatment groups. Alkaline phosphatase and collagen-I expressions were upregulated in MB + 2 J/cm(2) group at 7th and 14th days, respectively. These results may contribute to the low-dose PDT researches and understanding PBM effects on osteoblast behavior but further studies are needed since inappropriate conditions may lead to undesirable results for both therapies.
引用
收藏
页码:1847 / 1855
页数:9
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