Cycloheximide blocks TGF-β1-induced apoptosis in murine hepatocytes

被引:0
|
作者
Liao, JH [1 ]
Zhou, BH [1 ]
Chai, MQ [1 ]
Song, JG [1 ]
机构
[1] Chinese Acad Sci, Shanghai Inst Biochem, State Key Lab Mol Biol, Shanghai 200031, Peoples R China
来源
ACTA PHARMACOLOGICA SINICA | 2001年 / 22卷 / 02期
关键词
transforming growth factor beta; apoptosis; cycloheximide; ceramides;
D O I
暂无
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
AIM: To study the mechanism of transforming growth factor beta (1)-induced apoptosis in cultured hepatocytes. METHODS: DNA fragmentation and fluorescent microscopy were used to characterize cell apoptosis. Crystal violet staining was used to assess cell viability. Immunoblotting was used to detect Tak1, p53, and Bax. Dual luciferase assay was used to determine TGF-beta (1)-induced gene expression. Thin layer chromatography was used to examine ceramide level in AML12 cells. RESULTS: In response to TGF-beta (1) treatment, AML12 cells exhibited typical changes, which was characteristic of apoptosis, such as condensation of chromatin, disintegration of nuclei, and DNA fragmentation. TGF-beta (1)-induced apoptosis in AML12 cells was completely blocked in the presence of cycloheximide. The inhibitory effect of cycloheximide was accompanied with down-regulation of Tak1 expression and TGF-beta (1)-induced PAI-1 expression. TGF-beta (1) induced p53 expression but not Bax. No increase of ceramide was obeserved in TGF-beta (1)-induced apoptosis. CONCLUSION: TGF-beta (1)-induced apoptosis requires TGF-beta (1)-induced gene expression.
引用
收藏
页码:176 / 182
页数:7
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