Immunolocalization of Estrogen Receptor Alpha in Adult Female Rat Hippocampus

被引：0

作者：

Rai, Anju Lata ^{[1
]}

Jeswar, Usha ^{[2
]}

机构：

[1] ITS Dent Coll, Greater Noida, Uttar Pradesh, India

[2] AJ Inst Med Sci, Mangalore, Karnataka, India

来源：

INTERNATIONAL JOURNAL OF MORPHOLOGY | 2010年 / 28卷 / 02期

关键词：

Estrogen receptor-alpha; Estrogen receptor-beta; Brain; Hippocampus; Interneurons; Cornua ammonis; DENDRITIC SPINE DENSITY; MOUSE-BRAIN; ER-ALPHA; BETA; CELLS; LOCALIZATION;

D O I：

暂无

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

The present study was planned to demonstrate the detailed immunoreactive (IR) distribution pattern of estrogen receptors (ER) in hippocampus of 15 female rats, adult, female Wistar rats in estrous phase. 30 mu m thick setions of hippocampal region fixed (4% buffered paraformaldehyde) were obtained with cryostat. The sections were processed free- floating for immunolocalization of ER using, mouse monoclonal anti-ER-alpha antibody with PAP technique. The results showed presence of ER immunoreactive neurons in all the subfields of hippocampus with some variations. In cornua ammonis (CA) maximum ER positive (+ve) neurons were localized in CA3 region. Layer analysis showed maximum localization in the stratum oriens (SO) region. In other subfields and layers of CA the IR neurons were comparatively less in number. The morphological characters of all ER +ve neurons showed them to be interneurons both in CA as well as in Dentate gyrus (DG).

引用

页码：483 / 487

页数：5

共 22 条

[1]

Adams MM, 2002, J NEUROSCI, V22, P3608

[2]

BLAUSTEIN JD, 1989, ADV COMP ENV PHYSL, V3, P23

[3] OBSERVATIONS IN A PRELIMINARY OPEN TRIAL OF ESTRADIOL THERAPY FOR SENILE DEMENTIA-ALZHEIMERS TYPE [J].