Preparation Technologies of the Polymer-Based MONOLITH Material and Its Application as Stationary Phase of Affinity Chromatography for the Separation of Biological Macromolecules

In recent years, a newly emerging polymer-based MONOLITH material (also known as the monolithic column, continuous bed) has been developed rapidly and it has already been utilized widely in various fields. Due to its unique three. dimensional continuous interconnected porous structure in a single piece, polymer-based MONOLITH has attracted more and more attention and it is regarded as one of the major breakthroughs in separation and filtration techniques-Compared with silica-based MONOLITH material, polymer. based MONOLITH material has the advantages of simple preparation process, good biocompatibility, high chemical stability and easy regulation of surface chemical properties. Therefore, polymer. based MONOLITH has always done a better job as a stationary phase of affinity chromatography for separating and analyzing biological macromolecules. In this review, the preparation techniques and the newest development of the polymer. based MONOLITH material are summarized. In addition, its application as stationary phase of affinity chromatography for the separation of biological macromolecules in the last 5 years is described.