In vitro anaerobic biofilms of human colonic microbiota

被引：19

作者：

Sproule-Willoughby, K. M. ^{[1
]}

Stanton, M. Mark ^{[1
]}

Rioux, K. P. ^{[2
,3
]}

McKay, D. M. ^{[4
]}

Buret, A. G. ^{[1
,5
]}

Ceri, H. ^{[1
]}

机构：

[1] Univ Calgary, Dept Biol Sci, Biofilm Res Grp, Calgary, AB T2N 1N4, Canada

[2] Univ Calgary, Hlth Sci Ctr, Div Gastroenterol, Dept Med, Calgary, AB T2N 4N1, Canada

[3] Univ Calgary, Hlth Sci Ctr, Dept Microbiol & Infect Dis, Calgary, AB T2N 4N1, Canada

[4] Univ Calgary, Dept Physiol & Pharmacol, Gastrointestinal Res Grp, Calvin Phoebe & Joan Snyder Inst Infect Immun & I, Calgary, AB T2N 4N1, Canada

[5] Univ Calgary, Fac Med, Hlth Sci Ctr, Inflammat Res Network, Calgary, AB T2N 4N1, Canada

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2010年 / 83卷 / 03期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Anaerobic bacteria; Biofilms; Intestinal microbiota; 16S RIBOSOMAL-RNA; REAL-TIME PCR; BACTERIAL BIOFILMS; FECES; IDENTIFICATION; MICROFLORA; DIVERSITY; PRIMERS; DISEASE; GUT;

D O I：

10.1016/j.mimet.2010.09.020

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The human gastrointestinal tract hosts a complex community of microorganisms that grow as biofilms on the intestinal mucosa. These bacterial communities are not well characterized, although they are known to play an important role in human health. This study aimed to develop a model for culturing biofilms (surface-adherent communities) of intestinal microbiota. The model utilizes adherent mucosal bacteria recovered from colonic biopsies to create multi-species biofilms. Culture on selective media and confocal microscopy indicated the biofilms were composed of a diverse community of bacteria. Molecular analyses confirmed that several phyla were represented in the model, and demonstrated stability of the community over 96 h when cultured in the device. This model is novel in its use of a multi-species community of mucosal bacteria grown in a biofilm mode of growth. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：296 / 301

页数：6

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