A strain of highly pathogenic porcine reproductive and respiratory syndrome virus: genomic characterization, pathogenicity, and construction of an infectious full-length cDNA clone

被引：4

作者：

Wang, Shuangyun ^{[1
,2
]}

Liu, Yanling ^{[1
,2
]}

Yu, Linyang ^{[1
,2
]}

Liang, Tairun ^{[1
,2
]}

Zhang, Pengfei ^{[1
,2
]}

Dong, Jianguo ^{[3
]}

Zhang, Leyi ^{[1
,2
]}

Liang, Pengshuai ^{[1
,2
]}

Wang, Lei ^{[1
,2
]}

Xu, Zheng ^{[1
,2
]}

Song, Changxu ^{[1
,2
]}

机构：

[1] South China Agr Univ, Coll Anim Sci, Guangzhou 510642, Peoples R China

[2] South China Agr Univ, Natl Engn Ctr Swine Breeding Ind, Guangzhou 510642, Peoples R China

[3] Xinyang Agr & Forestry Univ, Sch Anim Husb & Med Engn, Xinyang 464000, Peoples R China

来源：

ARCHIVES OF VIROLOGY | 2021年 / 166卷 / 11期

关键词：

NORTH-AMERICAN TYPE; GENETIC DIVERSITY; PRRSV; GENERATION; PROTEIN; NSP2; IDENTIFICATION; EVOLUTION; VR-2332; DISEASE;

D O I：

10.1007/s00705-021-05212-w

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Porcine reproductive and respiratory syndrome (PRRS) is a highly contagious infectious disease caused by porcine reproductive and respiratory syndrome virus (PRRSV), which inflicts major economic losses on the global pig farming industry. Based on its similarity to highly pathogenic strains, the GDzj strain isolated in this study was predicted to be highly pathogenic. We therefore analyzed the pathogenicity of this strain experimentally in piglets. All piglets challenged with this virus experienced fever or high fever, loss of appetite, decreased food intake, daily weight loss, shortness of breath, and listlessness, and the necropsy results showed that they had experienced severe interstitial pneumonia. We then used the BAC system to construct a full-length cDNA infectious clone of GDzj, and the rescued virus displayed in vitro proliferation characteristics similar to those of the parental PRRSV strain. In summary, we successfully isolated a highly pathogenic PRRSV strain and constructed a full-length infectious cDNA clone from it, thereby providing an effective reverse genetics platform for further study of viral pathogenesis.

引用

页码：3127 / 3141

页数：15

共 36 条

[1] Ratification vote on taxonomic proposals to the International Committee on Taxonomy of Viruses (2016) [J].

Adams, Michael J. ;

Lefkowitz, Elliot J. ;

King, Andrew M. Q. ;

Harrach, Balazs ;

Harrison, Robert L. ;

Knowles, Nick J. ;

Kropinski, Andrew M. ;

Krupovic, Mart ;

Kuhn, Jens H. ;

Mushegian, Arcady R. ;

Nibert, Max ;

Sabanadzovic, Sead ;

Sanfacon, Helene ;

Siddell, Stuart G. ;

Simmonds, Peter ;

Varsani, Arvind ;

Zerbini, Francisco Murilo ;

Gorbalenya, Alexander E. ;

Davison, Andrew J. .

ARCHIVES OF VIROLOGY, 2016, 161 (10) :2921-2949

[2] CHARACTERIZATION OF SWINE INFERTILITY AND RESPIRATORY SYNDROME (SIRS) VIRUS (ISOLATE ATCC VR-2332) [J].

BENFIELD, DA ;

NELSON, E ;

COLLINS, JE ;

HARRIS, L ;

GOYAL, SM ;

ROBISON, D ;

CHRISTIANSON, WT ;

MORRISON, RB ;

GORCYCA, D ;

CHLADEK, D .

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 1992, 4 (02) :127-133

[3] INFECTIOUS TRANSCRIPTS AND CDNA CLONES OF RNA VIRUSES [J].

BOYER, JC ;

HAENNI, AL .

VIROLOGY, 1994, 198 (02) :415-426

[4]

Cavanagh D, 1997, ARCH VIROL, V142, P629

[5] Dynamics and evolution of highly pathogenic porcine reproductive and respiratory syndrome virus following its introduction into a herd concurrently infected with both types 1 and 2 [J].

Chaikhumwang, Puwich ;

Tantituvanont, Angkana ;

Tripipat, Thitima ;

Tipsombatboon, Pavita ;

Piriyapongsa, Jittima ;

Nilubol, Dachrit .

INFECTION GENETICS AND EVOLUTION, 2015, 30 :164-174

[6] High genetic diversity of Chinese porcine reproductive and respiratory syndrome viruses from 2016 to 2019 [J].

Chen, Nanhua ;

Xiao, Yanzhao ;

Ye, Mengxue ;

Li, Xinshuai ;

Li, Shubin ;

Xie, Ningjun ;

Wei, Yue ;

Wang, Jialin ;

Zhu, Jianzhong .

RESEARCH IN VETERINARY SCIENCE, 2020, 131 :38-42

[7]

Fang Y, 2006, ADV EXP MED BIOL, V581, P605

[8] A full-length cDNA infectious clone of North American type 1 porcine reproductive and respiratory syndrome virus: Expression of green fluorescent protein in the Nsp2 region [J].

Fang, Ying ;

Rowland, Raymond R. R. ;

Roof, Michael ;

Lunney, Joan K. ;

Christopher-Hennings, Jane ;

Nelson, Eric A. .

JOURNAL OF VIROLOGY, 2006, 80 (23) :11447-11455

[9] Porcine Respiratory and Reproductive Syndrome Virus Variants, Vietnam and China, 2007 [J].

Feng, Youjun ;

Zhao, Tiezhu ;

Nguyen, Tung ;

Inui, Ken ;

Ma, Ying ;

Nguyen, Thi Hoa ;

Nguyen, Van Cam ;

Liu, Di ;

Bui, Quang Anh ;

To, Long Thanh ;

Wang, Chuanbin ;

Tian, Kegong ;

Gao, George F. .

EMERGING INFECTIOUS DISEASES, 2008, 14 (11) :1774-1776

[10] The genetic diversity of European type PRRSV is similar to that of the North American type but is geographically skewed within Europe [J].

Forsberg, R ;

Storgaard, T ;

Nielsen, HS ;

Oleksiewicz, MB ;

Cordioli, P ;

Sala, G ;

Hein, J ;

Botnert, A .

VIROLOGY, 2002, 299 (01) :38-47

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