Nuclear envelope budding is a response to cellular stress

被引：26

作者：

Panagaki, Dimitra ^{[1
]}

Croft, Jacob T. ^{[1
]}

Keuenhof, Katharina ^{[1
]}

Berglund, Lisa Larsson ^{[1
]}

Andersson, Stefanie ^{[1
]}

Kohler, Verena ^{[2
]}

Buttner, Sabrina ^{[2
]}

Tamas, Markus J. ^{[1
]}

Nystrom, Thomas ^{[3
]}

Neutze, Richard ^{[1
]}

Hoog, Johanna L. ^{[1
]}

机构：

[1] Univ Gothenburg, Dept Chem & Mol Biol, S-40530 Gothenburg, Sweden

[2] Stockholm Univ, Wenner Gren Inst, Dept Mol Bioscienses, S-10691 Stockholm, Sweden

[3] Univ Gothenburg, Dept Microbiol & Immunol, S-40530 Gothenburg, Sweden

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2021年 / 118卷 / 30期

基金：

瑞典研究理事会; 奥地利科学基金会;

关键词：

nuclear transport; budding; vesicles; electron tomography; protein quality control; UBIQUITIN-PROTEASOME PATHWAY; PORE COMPLEX; QUALITY-CONTROL; PROTEIN AGGREGATION; HEAT-SHOCK; NUCLEOCYTOPLASMIC TRAFFICKING; CYTOSOLIC PROTEINS; YEAST; ELECTRON; CELLS;

D O I：

10.1073/pnas.2020997118

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Nuclear envelope budding (NEB) is a recently discovered alternative pathway for nucleocytoplasmic communication distinct from the movement of material through the nuclear pore complex. Through quantitative electron microscopy and tomography, we demonstrate how NEB is evolutionarily conserved from early protists to human cells. In the yeast Saccharomyces cerevisiae, NEB events occur with higher frequency during heat shock, upon exposure to arsenite or hydrogen peroxide, and when the proteasome is inhibited. Yeast cells treated with azetidine-2-carboxylic acid, a proline analog that induces protein misfolding, display the most dramatic increase in NEB, suggesting a causal link to protein quality control. This link was further supported by both localization of ubiquitin and Hsp104 to protein aggregates and NEB events, and the evolution of these structures during heat shock. We hypothesize that NEB is part of normal cellular physiology in a vast range of species and that in S. cerevisiae NEB comprises a stress response aiding the transport of protein aggregates across the nuclear envelope.

引用

页数：11

共 108 条

[1] NUCLEUS OF NOCTILUCA SCINTILLANS - ASPECTS OF NUCLEOCYTOPLASMIC EXCHANGES AND FORMATION OF NUCLEAR MEMBRANE [J].