Toxoplasma gondii and Neospora caninum in wild small mammals: Seroprevalence, DNA detection and genotyping

被引：28
作者：
Machacova, Tereza [1 ]
Ajzenberg, Daniel [3 ,4 ]
Zakovska, Alena [2 ]
Sedlak, Kamil [5 ]
Bartova, Eva [1 ]
机构：
[1] Univ Vet & Pharmaceut Sci Brno, Fac Vet Hyg & Ecol, Dept Biol & Wildlife Dis, Palackeho Tr 1946-1, Brno 61242, Czech Republic
[2] Masaryk Univ, Fac Sci, Dept Expt Biol, Kamenice 753-5, Brno 62500, Czech Republic
[3] Ctr Hosp Univ Dupuytren, Biol Resource Ctr, Ctr Natl Reference CNR Toxoplasmose Toxoplasma, F-87042 Limoges, France
[4] Univ Limoges, Fac Med, Lab Parasitol & Mycol, INSERM,UMR S 1094,Trop Neuroepidemiol, F-87025 Limoges, France
[5] State Vet Inst Prague, Dept Virol & Serol, Sidlistni 136-24, Prague, Czech Republic
来源：
VETERINARY PARASITOLOGY | 2016年 / 223卷
关键词：
Toxoplasmosis; Neosporosis; Rodents; Genotype; Czech Republic; RODENTS; PCR; STRAINS;
D O I：
10.1016/j.vetpar.2016.04.018
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Generally, rodents and other small mammals are considered as one of the sources of Toxoplasma gondii or Neospora caninum infection for cats and dogs as the definitive hosts of these two parasites, respectively. The aim of the study was to find out the prevalence of these two parasites in wild small mammals from the Czech Republic and to characterize T. gondii isolates by methods of molecular biology. A total of 621 wild small mammals were caught in the Czech Republic during years 2002-2014. Antibodies to T. gondii were detected by latex agglutination test in six (2.5%) of 240 small mammals (in two A. agrarius and four A. flavicollis). Antibodies to N. caninum were detected by commercially available competitive-inhibition enzyme-linked immunosorbent assay in one A. flavicolis (0.4%). Three of 427 (0.7%) liver samples were positive for T. gondii by PCR while negative for N. caninum. All embryo samples (n = 102) were negative for both T. gondii and N. caninum. The three liver samples positive for T. gondii DNA (two from A. flavicollis and one from A. sylvaticus) were genotyped by 15 microsatellite markers and characterized as type II. To our knowledge, this is the first information about genetic characterization of T. gondii isolates in small mammals from Europe and the first detection of N. caninum antibodies in wild rodents from the Czech Republic. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码：88 / 90
页数：3
相关论文
共 12 条
[1]   Genotyping of Toxoplasma gondii Isolates with 15 Microsatellite Markers in a Single Multiplex PCR Assay [J].
Ajzenberg, Daniel ;
Collinet, Frederic ;
Mercier, Aurelien ;
Vignoles, Philippe ;
Darde, Marie-Laure .
JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (12) :4641-4645
[2]   RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISMS AMONG TOXOPLASMA-GONDII STRAINS [J].
CRISTINA, N ;
OURY, B ;
AMBROISETHOMAS, P ;
SANTORO, F .
PARASITOLOGY RESEARCH, 1991, 77 (03) :266-268
[3]   Evidence of Neospora caninum DNA in wild rodents [J].
Ferroglio, E. ;
Pasino, M. ;
Romano, A. ;
Grande, D. ;
Pregel, P. ;
Trisciuogjio, A. .
VETERINARY PARASITOLOGY, 2007, 148 (3-4) :346-349
[4]   An innovative survey underlining the significant level of contamination by Toxoplasma gondii of ovine meat consumed in France [J].
Halos, Lenaig ;
Thebault, Anne ;
Aubert, Dominique ;
Thomas, Myriam ;
Perret, Catherine ;
Geers, Regine ;
Alliot, Annie ;
Escotte-Binet, Sandie ;
Ajzenberg, Daniel ;
Darde, Marie-Laure ;
Durand, Benoit ;
Boireau, Pascal ;
Villena, Isabelle .
INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2010, 40 (02) :193-200
[5]  
Hejlicek K, 1998, FOLIA ZOOL, V47, P93
[6]   Genetic characterisation of Toxoplasma gondii isolates from European beavers (Castor fiber) and European wildcats (Felis silvestris silvestris) [J].
Herrmann, D. C. ;
Wibbelt, G. ;
Goetz, M. ;
Conraths, F. J. ;
Schares, G. .
VETERINARY PARASITOLOGY, 2013, 191 (1-2) :108-111
[7]   TOXOPLASMA-GONDII COMPRISES 3 CLONAL LINEAGES - CORRELATION OF PARASITE GENOTYPE WITH HUMAN-DISEASE [J].
HOWE, DK ;
SIBLEY, LD .
JOURNAL OF INFECTIOUS DISEASES, 1995, 172 (06) :1561-1566
[8]   The role of rodents and shrews in the transmission of Toxoplasma gondii to pigs [J].
Kijlstra, Aize ;
Meerburg, Bastiaan ;
Cornelissen, Jan ;
De Craeye, Stephane ;
Vereijken, Pieter ;
Jongert, Erik .
VETERINARY PARASITOLOGY, 2008, 156 (3-4) :183-190
[9]   Restriction fragment length polymorphism and virulence of Czech Toxoplasma gondii strains [J].
Literak, I ;
Rychlik, I ;
Svobodova, V ;
Pospisil, Z .
INTERNATIONAL JOURNAL FOR PARASITOLOGY, 1998, 28 (09) :1367-1374
[10]   Neospora caninum and Toxoplasma gondii in brain tissue of feral rodents and insectivores caught on farms in the Netherlands [J].
Meerburg, B. G. ;
De Craeye, S. ;
Dierick, K. ;
Kijlstra, A. .
VETERINARY PARASITOLOGY, 2012, 184 (2-4) :317-320
12