Hydrogen regulates the M1/M2 polarization of alveolar macrophages in a rat model of chronic obstructive pulmonary disease

被引:13
作者
Su, Jing-chao [1 ]
Zhang, Yi [2 ,3 ]
Cheng, Chen [2 ,3 ]
Zhu, Yi-nan [1 ]
Ye, Yu-meng [1 ]
Sun, Yong-kang [1 ]
Xiang, Shui-ying [3 ]
Wang, Yuan [1 ]
Liu, Zi-bing [3 ,4 ]
Zhang, Xin-fang [1 ,4 ]
机构
[1] Anhui Univ Chinese Med, Coll Integrated Chinese & Western Med, Hefei 230038, Anhui, Peoples R China
[2] Anhui Univ Chinese Med, Grad Sch, Hefei, Anhui, Peoples R China
[3] Anhui Univ Chinese Med, Coll Acupuncture & Tuina, Hefei, Anhui, Peoples R China
[4] Anhui Univ Chinese Med, Key Lab Xinan Med, Minist Educ, Hefei, Anhui, Peoples R China
基金
中国国家自然科学基金;
关键词
hydrogen; chronic obstructive pulmonary disease; macrophage polarization; inflammation; ACUTE LUNG INJURY; MOLECULAR-HYDROGEN; OXIDATIVE STRESS; COPD; INFLAMMATION; INHALATION; ACTIVATION; EXPRESSION; PREVENTION; MANAGEMENT;
D O I
10.1080/01902148.2021.1919788
中图分类号
R56 [呼吸系及胸部疾病];
学科分类号
摘要
Objective Chronic obstructive pulmonary disease (COPD) is a respiratory disease with high morbidity and mortality worldwide, so far there is no ideal treatment method. Previous studies have shown that hydrogen (H-2) is involved in the treatment of COPD as an antioxidant. In this study, the effect of H-2 on M1/M2 polarization of alveolar macrophages in COPD rats was observed, and its anti-inflammatory mechanism was further elucidated. Methods: Twenty-four Sprague-Dawley rats were randomly divided into three groups including the control, COPD and H-2 group. A rat model of COPD was established by cigarette exposure combined with lipopolysaccharide (LPS) induction. H-2 therapy was administered 2 hours per day for 14 days. Lung function and pathology were assessed. The levels of interleukin (IL)-6, tumor necrosis factor (TNF)-alpha, transforming growth factor (TGF)-beta 1 and IL-10 in bronchoalveolar lavage fluid (BALF) and lung tissue were measured by enzyme-linked immunosorbent assay. The mRNA, protein expression and immunoreactivity of inducible nitric oxide synthase (iNOS) and arginase (Arg)-1 in lung were observed by quantitative real-time PCR, western blot and immunohistochemistry. Results: Compared with the control rats, there were a significant decline in lung function, a marked inflammatory infiltration and pulmonary parenchymal remodeling and the increases of IL-6, TNF-alpha and TGF-beta 1 levels in BALF and lung tissue, but a lower expression of IL-10 in COPD rats. The iNOS mRNA and protein expression, as well as its optical density (OD), were increased significantly in lung tissue, while those of Arg-1 decreased significantly. H-2 treatment improved the lung function and the parenchymal inflammation, reversed the increased levels of IL-6, TNF-alpha and TGF-beta 1, and the lower IL-10. Meanwhile, H-2 also down-regulated the expression of iNOS, but up-regulated expression of Arg-1 in lung tissue. Conclusion: H-2 reduces inflammation in the lung of COPD, which may be related to its inhibition of M1 type polarization and activation of M2 type polarization of alveolar macrophage.
引用
收藏
页码:301 / 310
页数:10
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