Stem cells from human exfoliated deciduous teeth (SHED) enhance wound healing and the possibility of novel cell therapy

被引：71

作者：

Nishino, Yudai ^{[2
]}

Yamada, Yoichi ^{[1
]}

Ebisawa, Katsumi ^{[3
]}

Nakamura, Sayaka ^{[2
]}

Okabe, Kazuto ^{[2
]}

Umemura, Eri ^{[2
]}

Hara, Kenji ^{[2
]}

Ueda, Minoru ^{[2
]}

机构：

[1] Nagoya Univ, Sch Med, Ctr Genet & Regenerat Med, Showa Ku, Nagoya, Aichi 4668550, Japan

[2] Nagoya Univ, Grad Sch Med, Dept Oral & Maxillofacial Surg, Nagoya, Aichi 4668550, Japan

[3] Nagoya Univ, Grad Sch Med, Dept Plast & Reconstruct Surg, Nagoya, Aichi 4668550, Japan

来源：

CYTOTHERAPY | 2011年 / 13卷 / 05期

基金：

日本学术振兴会;

关键词：

cell therapy; human mesenchymal stromal cells; hyaluronic acid; stem cells from human exfoliated deciduous teeth; wound healing; SOFT-TISSUE AUGMENTATION; BONE-MARROW; DENTAL-PULP; DERMAL FIBROBLASTS; ACID; DIFFERENTIATION; ANGIOGENESIS; HYALURONAN; DISEASE;

D O I：

10.3109/14653249.2010.542462

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Background aims. In recent years, stem cells from human exfoliated deciduous teeth (SHED) have received attention as a novel stem cell source with multipotent potential. We examined the effect on wound-healing promotion with unique stem cells from deciduous teeth as a medical waste. Methods. An excisional wound-splinting mouse model was used and the effect of wound healing among SHED, human mesenchymal stromal cells (hMSCs), human fibroblasts (hFibro) and a control (phosphate-buffered saline; PBS) was evaluated by macroscopy, histology and enzyme-linked immunosorbent assay (ELISA), and the expression of hyaluronan (HA), which is related to wound healing, investigated. Results. SHED and hMSCs accelerated wound healing compared with hFibro and the control. There was a statistically significant difference in wound healing area among hFibro, hMSCs and SHED compared with the control after day 5. At days 7 and 14 after cell transplantation, the histologic observation showed that transplanted PKH26-positive cells were surrounded by human HA binding protein, especially in hMSCs and SHED. HA expression volume values were 1558.41 +/- 60.33 (control), 2092.75 +/- 42.56 (hFibro), 2342.07 +/- 188.10 (hMSCs) and 2314.85 +/- 164.91 (SHED) ng/mg, respectively, and significantly higher in hMSCs and SHED compared with hFibro and control at days 7 and 14 (P < 0.05). Conclusions. Our results show that SHED hMSCs have similar effects of wound-healing promotion as hFibro and controls. This implies that SHED might offer a unique stem cell resource and the possibility of novel cell therapies for wound healing in the future.

引用

页码：598 / 605

页数：8