EFFECTOR OF TRANSCRIPTION2 is involved in xylem differentiation and includes a functional DNA single strand cutting domain

被引：12

作者：

Ivanov, Rumen ^{[1
]}

Tiedemann, Jens ^{[1
]}

Czihal, Andreas ^{[1
]}

Schallau, Anna ^{[1
]}

Diep, Le Hong ^{[1
]}

Mock, Hans-Peter ^{[1
]}

Claus, Bernhard ^{[1
]}

Tewes, Annegret ^{[1
]}

Baeumlein, Helmut ^{[1
]}

机构：

[1] Leibniz Inst Plant Genet & Crop Plant Res, IPK, D-06466 Gatersleben, Germany

来源：

DEVELOPMENTAL BIOLOGY | 2008年 / 313卷 / 01期

关键词：

gene regulation; xylem differentiation; transcription factors; single strand cutting; GIY-YIG domain;

D O I：

10.1016/j.ydbio.2007.09.061

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

EFFECTORS OF TRANSCRIPTION2 (ET) are plant-specific regulatory proteins characterized by the presence of two to five C-terminal DNA-and Zn-binding repeats, and a highly conserved cysteine pattern. We describe the structural characterization of the three member Arabidopsis thaliana ET gene family and reveal some allelic sequence polymorphisms. A mutation analysis showed that AtET2 affects the expression of various KNAT genes involved in the maintenance of the undifferentiated state of cambial meristem cells. It also plays a role in the regulation of GA5 (gibberellin 3-beta-dioxygenase) and the cell-cycle-related GASA4. A correlation was established between AtET2 expression and the cellular differentiation state. AtET-GFP fusion proteins shuttle between the cytoplasm and nucleus, with the AtET2 product prevented from entering the nucleus in non-differentiating cells. Within the nucleus, AtET2 probably acts via a single strand cutting domain. A more general regulatory role for ET factors is proposed, governing cell differentiation in cambial meristems, a crucial process for the development of plant vascular tissues. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：93 / 106

页数：14

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