Molecular cloning, tissue distribution and the expression of cystine/glutamate exchanger (xCT, SLC7A11) in different tissues during development in broiler chickens

被引：11

作者：

Choi, Janghan ^{[1
]}

Li, Weiqi ^{[1
]}

Schindell, Brayden ^{[1
]}

Ni, Liju ^{[1
,2
]}

Liu, Shangxi ^{[1
]}

Zhao, Xiaoya ^{[1
]}

Gong, Joshua ^{[3
]}

Nyachoti, Martin ^{[1
]}

Yang, Chengbo ^{[1
]}

机构：

[1] Univ Manitoba, Dept Anim Sci, Winnipeg, MB R3T 2N2, Canada

[2] Shanghai Lab Anim Res Ctr, Shanghai 201203, Peoples R China

[3] Agr Agri Food Canada, Guelph Res & Dev Ctr, Guelph, ON N1G 5C9, Canada

来源：

ANIMAL NUTRITION | 2020年 / 6卷 / 01期

关键词：

Amino acid; Broiler chickens; Cystine/glutamate transporter; Development; Gene expression; IMPROVES MEAT QUALITY; OXIDATIVE STRESS; SYSTEM X(C)(-); VITAMIN-E; ANTIOXIDANT STATUS; ALPHA-TOCOPHEROL; HEAT-STRESS; L-GLUTAMATE; ACID; CYSTINE;

D O I：

10.1016/j.aninu.2019.10.001

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The cystine/glutamate exchanger (xCT, SLC7A11) is a component of the system Xc amino-acid antiporter that is able to export glutamate and import cysteine into cells. The xCT amino acid exchanger has received a lot of attention, due to the fact that cysteine is an essential substrate for the synthesis of glutathione (GSH), an endogenous antioxidant in cells. The objective of this research was to clone the full-length cDNA of chicken xCT, and to investigate the gene expression of xCT in different tissues, including intestinal segments of broiler chickens during development. The full-length cDNA of chicken xCT (2,703 bp) was obtained from the jejunum by reverse transcription-PCR and sequenced. Homology tests showed that chicken xCT had 80.4%, 80.2%, and 71.2% homology at the nucleotide level with humans, cattle, and rats, respectively. Likewise, amino acid sequence analysis showed that chicken xCT protein is 86.4%, 79.3%, and 75.6% homologous with humans, cattle, and rats, respectively. Additionally, phylogenetic analysis indicated that chicken xCT genes share a closer genetic relationship with humans and cattle, than with rats. The chicken xCT protein has 12 transmembrane helixes, 6 extracellular loops, and 5 intracellular loops. The mRNA of xCT was detected in all tissues, including intestinal segments, in which the mRNA expression of xCT was significantly higher (P < 0.05) within the colon, compared to the jejunum and ileum. During development, a linear pattern of changes regarding the levels of the xCT mRNA was found, indicating that there was an abundance of xCT within the duodenum (P < 0.05). Furthermore, there were changes of the xCT mRNA abundance in the colon during development, which displayed linear and cubic patterns (P < 0.05). These results indicated that xCT is widely expressed both in intestinal segments, as well as other organs that are not associated with nutrient absorption. Further investigation is needed to characterize the functional relevance of xCT activity in oxidative stress and inflammation in the small intestine of broiler chickens. (c) 2020, Chinese Association of Animal Science and Veterinary Medicine. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

引用

页码：107 / 114

页数：8

共 57 条

[1]

[Anonymous], 1994, NUTRIENT REQUIREMENT

[2] Concentration dependent antioxidant/pro-oxidant activity of curcumin Studies from AAPH induced hemolysis of RBCs [J].