Atomic force microscopy reveals multiple patterns of antenna organization in purple bacteria: implications for energy transduction mechanisms and membrane modeling

被引:28
作者
Sturgis, James N. [2 ]
Niederman, Robert A. [1 ]
机构
[1] Rutgers State Univ, Dept Mol Biol & Biochem, Nelson Biol Labs, Piscataway, NJ 08854 USA
[2] CNRS, UPR 9027, Inst Biol Struct & Microbiol, Lab Ingn Syst Macromol, F-13402 Marseille, France
关键词
light harvesting complexes; membrane protein; Rhodobacter sphaeroides; Rhodospirillum photometricum;
D O I
10.1007/s11120-007-9239-0
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Recent topographs of the intracytoplasmic membrane (ICM) of purple bacteria obtained by atomic force microscopy (AFM) have provided the first surface views of the native architecture of a multicomponent biological membrane at submolecular resolution, representing an important landmark in structural biology. A variety of species-dependent, closely packed arrangements of light-harvesting (LH) complexes was revealed: the most highly organized was found in Rhodobacter sphaeroides in which the peripheral LH2 antenna was seen either in large clusters or in fixed rows interspersed among ordered arrays of dimeric LH1-reaction center (RC) core complexes. A more random organization was observed in other species containing both the LH1 and LH2 complexes, as typified by Rhododspirillum photometricum with randomly packed monomeric LH1-RC core complexes intermingled with large, paracrystalline domains of LH2 antenna. Surprisingly, no structures that could be identified as the ATP synthase or cytochrome bc(1) complexes were observed, which may reflect their localization at ICM vesicle poles or in curved membrane areas, out of view from the flat regions imaged by AFM. This possible arrangement of energy transducing complexes has required a reassessment of energy tranduction mechanisms which place the cytochrome bc(1) complex in close association with the RC. Instead, more plausible proposals must account for the movement of quinone redox species over considerable membrane distances on appropriate time scales. AFM, together with atomic resolution structures are also providing the basis for molecular modeling of the ICM that is leading to an improved picture of the supramolecular organization of photosynthetic complexes, as well as the forces that drive their segregation into distinct domains.
引用
收藏
页码:269 / 278
页数:10
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