Testing and validating a modified CTAB DNA extraction method to enable molecular parentage analysis of fertilized eggs and larvae of an emerging South African aquaculture species, the dusky kob Argyrosomus japonicus

被引：16

作者：

Mirimin, L. ^{[1
,2
]}

Roodt-Wilding, R. ^{[1
]}

机构：

[1] Univ Stellenbosch, Dept Genet, Mol Breeding & Biodivers Res Grp, ZA-7600 Stellenbosch, South Africa

[2] Galway Mayo Inst Technol, Marine & Freshwater Res Ctr, Dublin, Ireland

来源：

JOURNAL OF FISH BIOLOGY | 2015年 / 86卷 / 03期

关键词：

F1; first generation; genomic DNA isolation; Kabeljou; microsatellite loci; pedigree analyses; FISH EGGS; MICROSATELLITE MARKERS; LIFE-HISTORY; SCIAENIDAE; MULLOWAY; IDENTIFICATION; AMPLIFICATION; FISHERIES; EASTERN; PISCES;

D O I：

10.1111/jfb.12639

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

This study describes the successful implementation of a modified cetyltrimethyl ammonium bromide (CTAB) protocol to isolate genomic DNA and amplify 14 microsatellite markers from fertilized eggs and larvae of an emerging South African farmed marine fish species, the dusky kob Argyrosomus japonicus. To test and validate the efficiency of this method, genetic data were utilized to resolve parentage and kinship of first-generation (F1) offspring produced in mass-spawning events of wild broodstock fish in a commercial hatchery. (C) 2015 The Fisheries Society of the British Isles

引用

页码：1218 / 1223

页数：6