Oral vaccination with attenuated Salmonella encoding the Trichinella spiralis 43-kDa protein elicits protective immunity in BALB/c mice

被引:10
作者
Wang, Nan [1 ,2 ,3 ]
Wang, Jun-Yao [1 ,2 ,3 ]
Pan, Tian-Xu [1 ,2 ,3 ]
Jiang, Yan-Long [1 ,2 ,3 ]
Huang, Hai-Bin [1 ,2 ,3 ]
Yang, Wen-Tao [1 ,2 ,3 ]
Shi, Chun-Wei [1 ,2 ,3 ]
Wang, Jian-Zhong [1 ,2 ,3 ]
Wang, Dan [1 ,2 ,3 ]
Zhao, Dan-Dan [1 ,2 ,3 ]
Sun, Li-Min [1 ,2 ,3 ]
Yang, Gui-Lian [1 ,2 ,3 ]
Wang, Chun-Feng [1 ,2 ,3 ]
机构
[1] Jilin Agr Univ, Coll Vet Med, Changchun, Peoples R China
[2] Jilin Agr Univ, Jilin Prov Engn Res Ctr Anim Probiot, Jilin Prov Key Lab Anim Microecol & Hlthy Breed, Changchun, Peoples R China
[3] Jilin Agr Univ, Key Lab Anim Prod & Prod Qual Safety, Minist Educ, Changchun, Peoples R China
基金
中国国家自然科学基金;
关键词
Trichinella spiralis; 43-kDa protein; Attenuated Salmonella; Vaccine; Immunoprotection; ANTIGENS; INFECTION; CELLS; IMMUNIZATION; STAGE;
D O I
10.1016/j.actatropica.2021.106071
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
A vaccine against Trichinella spiralis infection is urgently needed to interrupt its transmission from domestic animals to humans. However, no vaccine against T. spiralis is currently available. Our previous study demonstrated that the use of the 43-kDa glycoprotein present in excretory-secretory (ES) proteins of muscle larvae (ML) as an intramuscular DNA vaccine led to a 52.1% protection rate against T. spiralis infection. Attenuated Salmonella strains have the advantage of eliciting mucosal immunity, which is important for controlling T. spiralis infections at the intestinal stage and can be provided as vaccines via oral or intranasal routes. Therefore, in this study, complete 43-kDa glycoprotein (Ts43) sequences of T. spiralis were cloned into the vector pYA3681, and the recombinant plasmid pYA3681-Ts43 was transformed into the attenuated Salmonella typhimurium strain chi 11802. The results showed that oral vaccination of mice with attenuated Salmonella carrying the recombinant plasmid pYA3681-Ts43 induced an evident elevation of the local intestinal mucosal sIgA and serum IgG antibody responses. The flow cytometry results showed that the percentages of CD4+ T cells and secreted IFN-gamma, IL-4, and IL17A in CD4+ T cells were significantly increased in the spleen and mesenteric lymph node (MLN) lymphocytes of the vaccinated groups. In addition, increased levels of the IFN-gamma, IL-4, and IL-17A cytokines were also observed in the serum of the immunized groups. The above immune response results in the immunized groups demonstrated that protective immunity was elicited in this study. Finally, vaccinated mice demonstrated a significant 45.9% reduction in ML burden after infection with T. spiralis. This study demonstrated that oral vaccination with Ts43 delivered by attenuated Salmonella elicited local and systemic concurrent Th1/Th2/Th17 immune responses and provided partial protection against T. spiralis infection in BALB/c mice. This is a prospective strategy for the prevention and control of trichinellosis.
引用
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页数:10
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共 51 条
[11]   Association between IL-17 and IgA in the joints of patients with inflammatory arthropathies [J].
Elicabe, Ricardo Javier ;
Silva, Juan Eduardo ;
Dave, Mabel Noemi ;
Lacoste, Maria Gabriela ;
Tamashiro, Hector ;
Blas, Rodrigo ;
Munarriz, Alicia ;
Rabinovich, Gabriel Adrian ;
Di Genaro, Maria Silvia .
BMC IMMUNOLOGY, 2017, 18
[12]   Th17: A New Participant in Gut Dysfunction in Mice Infected with Trichinella spiralis [J].
Fu, Yu ;
Wang, Wenfeng ;
Tong, Jingjing ;
Pan, Qi ;
Long, Yanqing ;
Qian, Wei ;
Hou, Xiaohua .
MEDIATORS OF INFLAMMATION, 2009, 2009
[13]   TH17 cells transdifferentiate into regulatory T cells during resolution of inflammation [J].
Gagliani, Nicola ;
Vesely, Maria Carolina Amezcua ;
Iseppon, Andrea ;
Brockmann, Leonie ;
Xu, Hao ;
Palm, Noah W. ;
de Zoete, Marcel R. ;
Licona-Limon, Paula ;
Paiva, Ricardo S. ;
Ching, Travers ;
Weaver, Casey ;
Zi, Xiaoyuan ;
Pan, Xinghua ;
Fan, Rong ;
Garmire, Lana X. ;
Cotton, Matthew J. ;
Drier, Yotam ;
Bernstein, Bradley ;
Geginat, Jens ;
Stockinger, Brigitta ;
Esplugues, Enric ;
Huber, Samuel ;
Flavell, Richard A. .
NATURE, 2015, 523 (7559) :221-U225
[14]   Helminth infections: the great neglected tropical diseases [J].
Hotez, Peter J. ;
Brindley, Paul J. ;
Bethony, Jeffrey M. ;
King, Charles H. ;
Pearce, Edward J. ;
Jacobson, Julie .
JOURNAL OF CLINICAL INVESTIGATION, 2008, 118 (04) :1311-1321
[15]   Impeded establishment of the infective stage of Trichinella in the intestinal mucosa of mice by passive transfer of an IgA monoclonal antibody [J].
Inaba, T ;
Sato, H ;
Kamiya, H .
JOURNAL OF VETERINARY MEDICAL SCIENCE, 2003, 65 (11) :1227-1231
[16]   Attenuated Salmonella typhimurium SV4089 as a Potential Carrier of Oral DNA Vaccine in Chickens [J].
Jazayeri, Seyed Davoud ;
Ideris, Aini ;
Zakaria, Zunita ;
Omar, Abdul Rahman .
JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY, 2012,
[17]   Survey of Trichinella infection from domestic pigs in the historical endemic areas of Henan province, central China [J].
Jiang, Peng ;
Zhang, Xi ;
Wang, Li Ang ;
Han, Lu Hong ;
Yang, Mei ;
Duan, Jiang Yang ;
Sun, Ge Ge ;
Qi, Xin ;
Liu, Ruo Dan ;
Wang, Zhong Quan ;
Cui, Jing .
PARASITOLOGY RESEARCH, 2016, 115 (12) :4707-4709
[18]   Comparison of Artificial Digestion and Baermann's Methods for Detection of Trichinella spiralis Pre-encapsulated Larvae in Muscles with Low-Level Infections [J].
Jiang, Peng ;
Wang, Zhong-Quan ;
Cui, Jing ;
Zhang, Xi .
FOODBORNE PATHOGENS AND DISEASE, 2012, 9 (01) :27-31
[19]   Protective immunity against Trichinella spiralis in mice elicited by oral vaccination with attenuated Salmonella-delivered TsSP1.2 DNA [J].
Li, Jie Feng ;
Guo, Kai Xia ;
Qi, Xin ;
Lei, Jun Jun ;
Han, Yue ;
Yan, Shu Wei ;
Jiang, Peng ;
Yu, Chuan ;
Cheng, Xiang Chao ;
Wang, Zhong Quan ;
Cui, Jing .
VETERINARY RESEARCH, 2018, 49
[20]   Oral vaccination of mice with Trichinella spiralis nudix hydrolase DNA vaccine delivered by attenuated Salmonella elicited protective immunity [J].
Liu, Pei ;
Wang, Zhong Quan ;
Liu, Ruo Dan ;
Jiang, Peng ;
Long, Shao Rong ;
Liu, Li Na ;
Zhang, Xin Zhuo ;
Cheng, Xiang Chao ;
Yu, Chuan ;
Ren, Hui Jun ;
Cui, Jing .
EXPERIMENTAL PARASITOLOGY, 2015, 153 :29-38