Exposure to a social stressor alters the structure of the intestinal microbiota: Implications for stressor-induced immunomodulation

被引:818
作者
Bailey, Michael T. [1 ,2 ]
Dowd, Scot E. [3 ,4 ]
Galley, Jeffrey D. [2 ]
Hufnagle, Amy R. [2 ]
Allen, Rebecca G. [5 ]
Lyte, Mark [6 ]
机构
[1] Ohio State Univ, Coll Med, Inst Behav Med Res, Columbus, OH 43210 USA
[2] Ohio State Univ, Coll Dent, Div Oral Biol, Columbus, OH 43210 USA
[3] Res & Testing Lab, Lubbock, TX 79407 USA
[4] Med Biofilm Res Inst, Lubbock, TX 79407 USA
[5] Ohio State Univ, Coll Med, Integrated Biomed Sci Grad Training Program, Columbus, OH 43210 USA
[6] Texas Tech Univ, Hlth Sci Ctr, Sch Pharm, Dept Pharm Practice, Lubbock, TX 79430 USA
关键词
Social stress; Gastrointestinal microbiota; Pyrosequencing; Inflammation; Immunomodulation; BACTERIAL TRANSLOCATION; INDUCED INCREASES; GUT MICROBIOTA; PSYCHOLOGICAL STRESS; IMMUNE-RESPONSES; LEAKY GUT; INTERLEUKIN-6; MICROFLORA; FLORA; COLONIZATION;
D O I
10.1016/j.bbi.2010.10.023
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The bodies of most animals are populated by highly complex and genetically diverse communities of microorganisms. The majority of these microbes reside within the intestines in largely stable but dynamically interactive climax communities that positively interact with their host. Studies from this laboratory have shown that stressor exposure impacts the stability of the microbiota and leads to bacterial translocation. The biological importance of these alterations, however, is not well understood. To determine whether the microbiome contributes to stressor-induced immunoenhancement, mice were exposed to a social stressor called social disruption (SDR), that increases circulating cytokines and primes the innate immune system for enhanced reactivity. Bacterial populations in the cecum were characterized using bacterial tag-encoded FLX amplicon pyrosequencing. Stressor exposure significantly changed the community structure of the microbiota, particularly when the microbiota were assessed immediately after stressor exposure. Most notably, stressor exposure decreased the relative abundance of bacteria in the genus Bacteroides, while increasing the relative abundance of bacteria in the genus Clostridium. The stressor also increased circulating levels of IL-6 and MCP-1, which were significantly correlated with stressor-induced changes to three bacterial genera (i.e., Coprococcus, Pseudobutyrivibrio, and Dorea). In follow up experiments, mice were treated with an antibiotic cocktail to determine whether reducing the microbiota would abrogate the stressor-induced increases in circulating cytokines. Exposure to SDR failed to increase IL-6 and MCP-1 in the antibiotic treated mice. These data show that exposure to SDR significantly affects bacterial populations in the intestines, and remarkably also suggest that the microbiota are necessary for stressor-induced increases in circulating cytokines. (C) 2010 Elsevier Inc. All rights reserved.
引用
收藏
页码:397 / 407
页数:11
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