Optimisation of the dehydrogenase assay for measurement of indigenous microbial activity in beach sediments contaminated with petroleum

被引：36
作者：
Mathew, M
Obbard, JP
机构：
[1] Natl Univ Singapore, Trop Marine Sci Inst, Singapore 119260, Singapore
[2] Natl Univ Singapore, Dept Chem & Environm Engn, Singapore 119260, Singapore
来源：
BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 03期
关键词：
beach sediments; dehydrogenase; hydrocarbon; redox measurements; tetrazolium salts;
D O I：
10.1023/A:1005613406948
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A dehydrogenase enzyme assay has been developed to measure microbial activity in petroleum-contaminated beach sediments. The assay, using 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazoliumchloride (INT) as the substrate provides a reliable measurement of overall indigenous microbiological activity in beach sediments containing similar to7% (w/w) oil. The method has been effectively used in field studies (Mathew et al. 1999) to monitor microbial activity in conjunction with the biodegradation of petroleum hydrocarbons.
引用
收藏
页码：227 / 230
页数：4
相关论文
共 14 条
[1]  
BITTON G, 1986, TOXICITY TESTING USI, P27
[2]   SOIL MICROBIAL ACTIVITY AS AN INDICATOR OF SOIL FERTILITY - LONG-TERM EFFECTS OF MUNICIPAL SEWAGE-SLUDGE ON AN ARID SOIL [J].
BRENDECKE, JW ;
AXELSON, RD ;
PEPPER, IL .
SOIL BIOLOGY & BIOCHEMISTRY, 1993, 25 (06) :751-758
[3]   COMPARISON AND IMPROVEMENT OF METHODS FOR DETERMINING SOIL DEHYDROGENASE-ACTIVITY BY USING TRIPHENYLTETRAZOLIUM CHLORIDE AND IODONITROTETRAZOLIUM CHLORIDE [J].
FRIEDEL, JK ;
MOLTER, K ;
FISCHER, WR .
BIOLOGY AND FERTILITY OF SOILS, 1994, 18 (04) :291-296
[4]   IMPROVED EXTRACTION OF IODONITROTETRAZOLIUM-FORMAZAN FROM SOIL WITH DIMETHYLFORMAMIDE [J].
GRIFFITHS, BS .
SOIL BIOLOGY & BIOCHEMISTRY, 1989, 21 (01) :179-180
[5]   BIOMASS AND ACTIVITY OF MICROORGANISMS IN A FUEL-OIL CONTAMINATED SOIL [J].
JOERGENSEN, RG ;
SCHMAEDEKE, F ;
WINDHORST, K ;
MEYER, B .
SOIL BIOLOGY & BIOCHEMISTRY, 1995, 27 (09) :1137-1143
[6]   Measurement of ammonia inhibition of microbial activity in biological wastewater treatment process using dehydrogenase assay [J].
Lee, SM ;
Jung, JY ;
Chung, YC .
BIOTECHNOLOGY LETTERS, 2000, 22 (12) :991-994
[7]   Monitoring of bioremediation by soil biological activities [J].
Margesin, R ;
Zimmerbauer, A ;
Schinner, F .
CHEMOSPHERE, 2000, 40 (04) :339-346
[8]   Low-temperature bioremediation of a waste water contaminated with anionic surfactants and fuel oil [J].
Margesin, R ;
Schinner, F .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1998, 49 (04) :482-486
[9]   Bioremediation of oil contaminated beach sediments using indigenous microorganisms in Singapore [J].
Mathew, M ;
Obbard, JP ;
Ting, YP ;
Gin, YH ;
Tan, HM .
ACTA BIOTECHNOLOGICA, 1999, 19 (03) :225-233
[10]   AEROBIC STEPWISE HYDROCARBON DEGRADATION AND FORMATION OF BIOSURFACTANTS BY AN ORIGINAL SOIL POPULATION IN A STIRRED REACTOR [J].
OBERBREMER, A ;
MULLERHURTIG, R .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1989, 31 (5-6) :582-586
12