Artocarpin is a polyspecific jacalin-related lectin with a monosaccharide preference for mannose

被引：20
作者：
Barre, A
Peumans, WJ
Rossignol, M
Borderies, G
Culerrier, R
Van Damme, EJM
Rougé, P
机构：
[1] CNRS, UMR 5546, F-31326 Castanet Tolosan, France
[2] Univ Ghent, Dept Mol Biotechnol, B-9000 Ghent, Belgium
来源：
BIOCHIMIE | 2004年 / 86卷 / 9-10期
关键词：
artocarpin; jacalin-related lectins; carbohydrate-binding specificity; polyspecificity;
D O I：
10.1016/j.biochi.2004.09.001
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A reinvestigation of the carbohydrate-binding properties revealed that artocarpin, a previously described mannose-specific lectin from jackfruit (Artocarpus integrifolia) seeds, behaves as a polyspecific lectin. Surface plasmon resonance hapten inhibition experiments demonstrated that artocarpin readily interacted with a wide range of monosaccharides covering galactose, N-acetylgalactosamine, mannose, glucose, sialic acid and N-acetylmuramic acid. Molecular docking confirmed this unexpected ability of artocarpin to interact with structurally different sugars. The biological significance of the polyspecificity of the lectin is discussed in terms of the broadening of the range of potential target glycans present on the surface of plant phytopathogens or predators. (C) 2004 Elsevier SAS. All rights reserved.
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收藏
页码：685 / 691
页数：7
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