Proteomic profiling of plasma in Huntington's disease reveals neuroinflammatory activation and biomarker candidates

被引：174

作者：

Dalrymple, Annette

Wild, Edward J.

Joubert, Richard

Sathasivam, Kirupa

Bjorkqvist, Maria

Petersen, Asa

Jackson, Graham S.

Isaacs, Jeremy D.

Kristiansen, Mark

Bates, Gillian P.

Leavitt, Blair R.

Keir, Geoff

Ward, Malcolm

Tabrizi, Sarah J.

机构：

[1] Natl Hosp Neurol & Neurosurg, Inst Neurol, MRC, Prion Unit, London WC1N 3BG, England

[2] Proteome Sci PLC, Surrey, England

[3] Inst Neurol, Dept Neurodegenerat, London WC1N 3BG, England

[4] Kings Coll London, Guys Hosp, Sch Med, London WC2R 2LS, England

[5] Wallenberg Neurosci Ctr, Dept Expt Med Sci, Neuronal Survival Unit, Lund, Sweden

[6] Univ British Columbia, Ctr Mol Med & Therapeut, Vancouver, BC V5Z 1M9, Canada

[7] Natl Hosp Neurol & Neurosurg, Neuroimmunol Lab, London WC1N 3BG, England

来源：

JOURNAL OF PROTEOME RESEARCH | 2007年 / 6卷 / 07期

基金：

英国医学研究理事会; 英国惠康基金;

关键词：

Huntington's disease; plasma; neuroinflammation; proteomics; 2D electrophoresis; LC/MS/MS; MALDI-TOF; ELISA; biomarkers;

D O I：

10.1021/pr0700753

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Huntington's disease (HD) causes widespread CNS changes and systemic abnormalities including endocrine and immune dysfunction. HD biomarkers are needed to power clinical trials of potential treatments. We used multiplatform proteomic profiling to reveal plasma changes with HD progression. Proteins of interest were evaluated using immunoblotting and ELISA in plasma from 2 populations, CSF and R6/2 mice. The identified proteins demonstrate neuroinflammation in HD and warrant further investigation as possible biomarkers.

引用

页码：2833 / 2840

页数：8