Neutrophil elastase is associated with serglycin on its way to lysosomes in U937 cells

Mutations in the neutrophil elastase (NE) gene have been postulated to interfere with normal intracellular trafficking of NE as all AP3-interacting membrane integrated protein and to cause severe congenital or cyclic neutropenia ill humans. Here, we show that in U937 promonocytes NE is synthesized as a predominantly soluble proenzyme and is completely secreted in the presence of phorbol esters similarly to serglycin. Using chemical cross-linking NE is shown to be associated with serglycin as 34 kDa proenzyme in the trans-Golgi region of these cells indicating that it is delivered to lysosomes associated with serglycin. (C) 2007 Elsevier Inc. All rights reserved.