Blood sampling using "dried blood spot": a clinical biology revolution underway?

被引：3

作者：

Hirtz, Christophe ^{[1
,2
,3
]}

Lehmann, Sylvain ^{[1
,2
,3
]}

机构：

[1] Hop St Eloi, CHU, Inst Rech Biotherapie, Montpellier, France

[2] Univ Montpellier I, CCBHM, Lab Biochim Clin Proteom, Montpellier, France

[3] INSERM, U1040, F-34000 Montpellier, France

来源：

ANNALES DE BIOLOGIE CLINIQUE | 2015年 / 73卷 / 01期

关键词：

dried blood spot; pre-analytical; PCR; mass spectrometry; ELISA; multiplex; TANDEM MASS-SPECTROMETRY; FILTER-PAPER BLOOD; HEPATITIS-C VIRUS; HUMAN CHORIONIC-GONADOTROPIN; MINIMALLY INVASIVE METHOD; IMMUNOREACTIVE-TRYPSIN; LIQUID-CHROMATOGRAPHY; ENZYME-IMMUNOASSAY; CYSTIC-FIBROSIS; ANTIBODIES;

D O I：

10.1684/abc.2014.1007

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Blood testing using the dried blood spot (DBS) is used since the 1960s in clinical analysis, mainly within the framework of the neonatal screening (Guthrie test). Since then numerous analytes such as nucleic acids, small molecules or lipids, were successfully measured on the DBS. While this pre-analytical method represents an interesting alternative to classic blood sampling, its use in routine is still limited. We review here the different clinical applications of the blood sampling on DBS and estimate its future place, supported by the new methods of analysis as the LC-MS mass spectrometry.

引用

页码：25 / 37

页数：13

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