Structure and distribution of a broadly expressed atypical sodium channel

被引：56

作者：

Akopian, AN ^{[1
]}

Souslova, V ^{[1
]}

Sivilotti, L ^{[1
]}

Wood, JN ^{[1
]}

机构：

[1] UCL, DEPT ANAT & DEV BIOL, LONDON WC1E 6BT, ENGLAND

来源：

FEBS LETTERS | 1997年 / 400卷 / 02期

基金：

英国惠康基金;

关键词：

dorsal root ganglion; voltage-gated sodium channel; cDNA cloning;

D O I：

10.1016/S0014-5793(96)01389-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA clone isolated from a rat dorsal root ganglion library encodes a 195 kDa voltage-gated sodium channel-like protein (SCL-11) with homology to the mouse (87%) and human (72%) atypical Na+ channels and rat partial clone NaG (98%). Two dominant mRNAs of 4.5 and 7 kb are expressed. The transcripts are present in lung, Schwann cells, pituitary and tissues containing smooth muscle cells. No functional channels could be detected on oocyte injection with cRNA, consistent with the absence of structural features necessary for voltage-gated sodium channel activity.

引用

收藏

页码：183 / 187

页数：5

相关论文

共 22 条

[1] PERIPHERAL NERVOUS SYSTEM-SPECIFIC GENES IDENTIFIED BY SUBTRACTIVE CDNA CLONING [J].

AKOPIAN, AN ;

WOOD, JN .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (36) :21264-21270

[2] A tetrodotoxin-resistant voltage-gated sodium channel expressed by sensory neurons [J].

Akopian, AN ;

Sivilotti, L ;

Wood, JN .

NATURE, 1996, 379 (6562) :257-262

[3] CLONING OF A SODIUM-CHANNEL ALPHA-SUBUNIT FROM RABBIT SCHWANN-CELLS [J].

BELCHER, SM ;

ZERILLO, CA ;

LEVENSON, R ;

RITCHIE, JM ;

HOWE, JR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (24) :11034-11038

[4] CELLULAR AND MOLECULAR-BIOLOGY OF VOLTAGE-GATED SODIUM-CHANNELS [J].

CATTERALL, WA .

PHYSIOLOGICAL REVIEWS, 1992, 72 (04) :S15-S48

[5] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].

CHOMCZYNSKI, P ;

SACCHI, N .

ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159

[6]

FELIPE A, 1994, J BIOL CHEM, V269, P30125

[7] THE GLIAL VOLTAGE-GATED SODIUM-CHANNEL - CELL-SPECIFIC AND TISSUE-SPECIFIC MESSENGER-RNA EXPRESSION [J].

GAUTRON, S ;

DOSSANTOS, G ;

PINTOHENRIQUE, D ;

KOULAKOFF, A ;

GROS, F ;

BERWALDNETTER, Y .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (15) :7272-7276

[8] MOLECULAR-CLONING OF AN ATYPICAL VOLTAGE-GATED SODIUM-CHANNEL EXPRESSED IN HUMAN HEART AND UTERUS - EVIDENCE FOR A DISTINCT GENE FAMILY [J].

GEORGE, AL ;

KNITTLE, TJ ;

TAMKUN, MM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (11) :4893-4897

[9]

GUY HR, 1990, TRENDS NEUROSCI, V13, P201

[10]

HEINEMANN SH, 1992, NATURE, V356, P411