Integration of banana streak badnavirus into the Musa genome:: Molecular and cytogenetic evidence

被引：143

作者：

Harper, G ^{[1
]}

Osuji, JO ^{[1
]}

Heslop-Harrison, JS ^{[1
]}

Hull, R ^{[1
]}

机构：

[1] John Innes Ctr Plant Sci Res, Norwich NR4 7UH, Norfolk, England

来源：

VIROLOGY | 1999年 / 255卷 / 02期

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1006/viro.1998.9581

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Breeding and tissue culture of certain cultivars of bananas (Musa) have red to high levels of banana streak badnavirus (BSV) infection in progeny from symptomless parents. BSV DNA hybridized to genomic DNA of one such parent, Obino l'Ewai, suggesting integration of Viral sequences. Sequencing of crones of Obino l'Ewai genomic DNA revealed an interface between BSV and Musa sequences and a complex BSV integrant. In situ hybridization revealed two different BSV sequence locations in Obino l'Ewai chromosomes and a complex arrangement of BSV and Muse sequences was shown by probing stretched DNA fibers. This is the first report of integrated sequences that possibly lead to a plant pararetrovirus episomal infection by a mechanism differing markedly from animal retroviral systems. (C) 1999 Academic Press.

引用

页码：207 / 213

页数：7

共 22 条

[1] Integration of multiple repeats of geminiviral DNA into the nuclear genome of tobacco during evolution [J].

Bejarano, ER ;

Khashoggi, A ;

Witty, M ;

Lichtenstein, C .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (02) :759-764

[2] Multiple repetitive DNA sequences in the paracentromeric regions of Arabidopsis thaliana L. [J].

Brandes, A ;

Thompson, H ;

Dean, C ;

HeslopHarrison, JS .

CHROMOSOME RESEARCH, 1997, 5 (04) :238-246

[3]

Diekmann M, 1996, FAO IPGRI TECHNICAL

[4] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].

FEINBERG, AP ;

VOGELSTEIN, B .

ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13

[5] High-resolution physical mapping in Arabidopsis thaliana and tomato by fluorescence in situ hybridization to extended DNA fibres [J].

Fransz, PF ;

AlonsoBlanco, C ;

Liharska, TB ;

Peeters, AJM ;

Zabel, P ;

deJong, JH .

PLANT JOURNAL, 1996, 9 (03) :421-430

[6]

Gawel N.J., 1991, PLANT MOL BIOL REP, V9, P262, DOI DOI 10.1007/BF02672076

[7] Cloning and sequence analysis of banana streak virus DNA [J].

Harper, G ;

Hull, R .

VIRUS GENES, 1998, 17 (03) :271-278

[8]

HARPER G, 1996, MUSAFRICA NEWSLETTER, V10, P128

[9] The chromosomal distributions of Ty1-copia group retrotransposable elements in higher plants and their implications for genome evolution [J].

Heslop-Harrison, JS ;

Brandes, A ;

Taketa, S ;

Schmidt, T ;

Vershinin, AV ;

Alkhimova, EG ;

Kamm, A ;

Doudrick, RL ;

Schwarzacher, T ;

Katsiotis, A ;

Kubis, S ;

Kumar, A ;

Pearce, SR ;

Flavell, AJ ;

Harrison, GE .

GENETICA, 1997, 100 (1-3) :197-204

[10] MOLECULAR-BIOLOGY OF VIRAL AND NONVIRAL RETROELEMENTS [J].

HULL, R ;

WILL, H .

TRENDS IN GENETICS, 1989, 5 (11) :357-359

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