FlyWire: online community for whole-brain connectomics

被引：102

作者：

Dorkenwald, Sven ^{[1
,2
]}

McKellar, Claire E. ^{[1
]}

Macrina, Thomas ^{[1
,2
]}

Kemnitz, Nico ^{[1
]}

Lee, Kisuk ^{[1
,3
]}

Lu, Ran ^{[1
]}

Wu, Jingpeng ^{[1
]}

Popovych, Sergiy ^{[1
,2
]}

Mitchell, Eric ^{[1
]}

Nehoran, Barak ^{[1
,2
]}

Jia, Zhen ^{[1
,2
]}

Bae, J. Alexander ^{[1
,4
]}

Mu, Shang ^{[1
]}

Ih, Dodam ^{[1
]}

Castro, Manuel ^{[1
]}

Ogedengbe, Oluwaseun ^{[1
]}

Halageri, Akhilesh ^{[1
]}

Kuehner, Kai ^{[1
]}

Sterling, Amy R. ^{[1
]}

Ashwood, Zoe ^{[1
,2
]}

Zung, Jonathan ^{[1
,2
]}

Brittain, Derrick ^{[5
]}

Collman, Forrest ^{[5
]}

Schneider-Mizell, Casey ^{[5
]}

Jordan, Chris ^{[1
]}

Silversmith, William ^{[1
]}

Baker, Christa ^{[1
]}

Deutsch, David ^{[1
]}

Encarnacion-Rivera, Lucas ^{[1
]}

Kumar, Sandeep ^{[1
]}

Burke, Austin ^{[1
]}

Bland, Doug ^{[1
]}

Gager, Jay ^{[1
]}

Hebditch, James ^{[1
]}

Koolman, Selden ^{[1
]}

Moore, Merlin ^{[1
]}

Morejohn, Sarah ^{[1
]}

Silverman, Ben ^{[1
]}

Willie, Kyle ^{[1
]}

Willie, Ryan ^{[1
]}

Yu, Szi-chieh ^{[1
]}

Murthy, Mala ^{[1
]}

Seung, H. Sebastian ^{[1
,2
]}

机构：

[1] Princeton Univ, Princeton Neurosci Inst, Princeton, NJ 08544 USA

[2] Princeton Univ, Comp Sci Dept, Princeton, NJ 08544 USA

[3] MIT, Brain & Cognit Sci Dept, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[4] Princeton Univ, Elect Engn Dept, Princeton, NJ 08544 USA

[5] Allen Inst Brain Sci, Seattle, WA USA

来源：

NATURE METHODS | 2022年 / 19卷 / 01期

基金：

美国国家卫生研究院;

关键词：

DROSOPHILA; SONG; BEHAVIOR; CIRCUIT; NEURONS; SYSTEM; CUES;

D O I：

10.1038/s41592-021-01330-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

FlyWire is an online community and a platform for proofreading electron microscopy-based connectome data of the Drosophila brain. Due to advances in automated image acquisition and analysis, whole-brain connectomes with 100,000 or more neurons are on the horizon. Proofreading of whole-brain automated reconstructions will require many person-years of effort, due to the huge volumes of data involved. Here we present FlyWire, an online community for proofreading neural circuits in a Drosophila melanogaster brain and explain how its computational and social structures are organized to scale up to whole-brain connectomics. Browser-based three-dimensional interactive segmentation by collaborative editing of a spatially chunked supervoxel graph makes it possible to distribute proofreading to individuals located virtually anywhere in the world. Information in the edit history is programmatically accessible for a variety of uses such as estimating proofreading accuracy or building incentive systems. An open community accelerates proofreading by recruiting more participants and accelerates scientific discovery by requiring information sharing. We demonstrate how FlyWire enables circuit analysis by reconstructing and analyzing the connectome of mechanosensory neurons.

引用

页码：119 / +

页数：24

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