High-resolution, noninvasive longitudinal live imaging of immune responses

被引：70

作者：

Abdulreda, Midhat H. ^{[1
]}

Faleo, Gaetano ^{[1
]}

Molano, Ruth Damaris ^{[1
]}

Lopez-Cabezas, Maite ^{[1
]}

Molina, Judith ^{[1
,3
]}

Tan, Yaohong ^{[6
]}

Echeverria, Oscar A. Ron ^{[1
]}

Zahr-Akrawi, Elsie ^{[1
]}

Rodriguez-Diaz, Rayner ^{[1
]}

Edlund, Patrick K. ^{[8
]}

Leibiger, Ingo ^{[8
]}

Bayer, Allison L. ^{[1
,4
]}

Perez, Victor ^{[4
,6
]}

Ricordi, Camillo ^{[1
,2
,3
,4
,7
,8
]}

Caicedo, Alejandro ^{[1
,3
,5
,7
]}

Pileggi, Antonello ^{[1
,2
,4
,7
]}

Berggren, Per-Olof ^{[1
,8
]}

机构：

[1] Univ Miami, Diabet Res Inst, Miami, FL 33136 USA

[2] Univ Miami, Dept Biomed Engn, Miami, FL 33136 USA

[3] Univ Miami, Miller Sch Med, Dept Med, Miami, FL 33136 USA

[4] Univ Miami, Miller Sch Med, Dept Microbiol & Immunol, Miami, FL 33136 USA

[5] Univ Miami, Miller Sch Med, Dept Physiol & Biophys, Miami, FL 33136 USA

[6] Univ Miami, Miller Sch Med, Bascom Palmer Eye Inst, Miami, FL 33136 USA

[7] Univ Miami, Miller Sch Med, DeWitt Daughtry Dept Surg, Div Cellular Transplantat, Miami, FL 33136 USA

[8] Karolinska Inst, Rolf Luft Res Ctr Diabet & Endocrinol, SE-17176 Stockholm, Sweden

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2011年 / 108卷 / 31期

基金：

瑞典研究理事会; 美国国家卫生研究院;

关键词：

ANTIGEN-PRESENTING CELLS; IN-VIVO; ANTERIOR-CHAMBER; T-CELLS; DEVIATION ACAID; ISLET CELLS; RECEPTOR; EFFECTOR; CCR5; ANTAGONIST;

D O I：

10.1073/pnas.1105002108

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Intravital imaging emerged as an indispensible tool in biological research, and a variety of imaging techniques have been developed to noninvasively monitor tissues in vivo. However, most of the current techniques lack the resolution to study events at the single-cell level. Although intravital multiphoton microscopy has addressed this limitation, the need for repeated noninvasive access to the same tissue in longitudinal in vivo studies remains largely unmet. We now report on a previously unexplored approach to study immune responses after transplantation of pancreatic islets into the anterior chamber of the mouse eye. This approach enabled (i) longitudinal, noninvasive imaging of transplanted tissues in vivo; (ii) in vivo cytolabeling to assess cellular phenotype and viability in situ; (iii) local intervention by topical application or intraocular injection; and (iv) real-time tracking of infiltrating immune cells in the target tissue.

引用

页码：12863 / 12868

页数：6

共 43 条

[1] Chemokines in islet allograft rejection [J].