Rare cancer cell analyzer for whole blood applications: Automated nucleic acid purification in a microfluidic disposable card

被引：14

作者：

Kokoris, M ^{[1
]}

Nabavi, M ^{[1
]}

Lancaster, C ^{[1
]}

Clemmens, J ^{[1
]}

Maloney, P ^{[1
]}

Capadanno, J ^{[1
]}

Gerdes, J ^{[1
]}

Battrell, CF ^{[1
]}

机构：

[1] Micronics Inc, Redmond, WA 98052 USA

来源：

METHODS | 2005年 / 37卷 / 01期

关键词：

rare cell; nucleic acid purification; microfluidic card;

D O I：

10.1016/j.ymeth.2005.07.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

One current challenge facing point-of-care cancer detection is that existing methods make it difficult, time consuming and too costly to (1) collect relevant cell types directly from a patient sample, such as blood and (2) rapidly assay those cell types to determine the presence or absence of a particular type of cancer. We present a proof of principle method for an integrated, sample-to-result, point-of-care detection device that employs microfluidics technology, accepted assays, and a silica membrane for total RNA purification on a disposable, credit card sized laboratory-on-card ("lab card") device in which results are obtained in minutes. Both yield and quality of on-card purified total RNA, as determined by both LightCycler and standard reverse transcriptase amplification of G6PDH and BCR-ABL transcripts, were found to be better than or equal to accepted standard purification methods. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：114 / 119

页数：6

共 10 条

[1] Nucleic acid purification using microfabricated silicon structures [J].