Duplication of insertion element IS50 associated with Tn5 transposition in Azospirillum brasilense

被引：0

作者：

Tripathi, AK ^{[1
]}

Tripathi, R

Ganguli, A

Bazzicalupo, M

机构：

[1] Banaras Hindu Univ, Fac Sci, Sch Biotechnol, Lab Bacterial Genet, Varanasi 221005, Uttar Pradesh, India

[2] Univ Florence, Dept Anim Biol & Genet, I-50125 Florence, Italy

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 1998年 / 44卷 / 11期

关键词：

Tn5; IS50; Azospirillum brasilense; recombinant DNA;

D O I：

10.1139/cjm-44-11-1110

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The characterization of a DNA fragment with a Tn5 insertion in a regulatory nif gene of Azospirillum brasilense is reported. Restriction endonuclease mapping, Southern hybridization with a Tn5 probe, and nucleotide sequencing revealed that IS50 had duplicated in Tn5. The duplication of an IS50 element suggests the occurrence of a replicative mechanism of transposition. A strategy, based on the bacterial ability of homologous recombination that was used to precisely eliminate Tn5 along with the duplicated IS50 element, is presented.

引用

收藏

页码：1110 / 1113

页数：4

相关论文

共 20 条

[1] A COMPARISON OF INTRAMOLECULAR REARRANGEMENTS PROMOTED BY TRANSPOSONS TN5 AND TN10 [J].

AHMED, A .

PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1991, 244 (1309) :1-9

[2] EVIDENCE FOR REPLICATIVE TRANSPOSITION OF TN5 AND TN9 [J].

AHMED, A .

JOURNAL OF MOLECULAR BIOLOGY, 1986, 191 (01) :75-84

[3] EXCISION AND TRANSPOSITION OF TN5 UPON INSERTION IN THE HHA GENE OF ESCHERICHIA-COLI [J].

BADENAS, C ;

MADRID, C ;

JUAREZ, A .

CANADIAN JOURNAL OF MICROBIOLOGY, 1994, 40 (07) :597-601

[4]

Berg D. E., 1989, M ohile DNA, P185

[5] THE USE OF TRANSPOSON TN5 MUTAGENESIS IN THE RAPID GENERATION OF CORRELATED PHYSICAL AND GENETIC MAPS OF DNA SEGMENTS CLONED INTO MULTICOPY PLASMIDS - A REVIEW [J].

DEBRUIJN, FJ ;

LUPSKI, JR .

GENE, 1984, 27 (02) :131-149

[6]

DEBRUIJN FJ, 1987, METHOD ENZYMOL, V154, P175

[7] CHARACTERIZATION OF THE TN5 TRANSPOSASE AND INHIBITOR PROTEINS - A MODEL FOR THE INHIBITION OF TRANSPOSITION [J].

DELACRUZ, NB ;

WEINREICH, MD ;

WIEGAND, TW ;

KREBS, MP ;

REZNIKOFF, WS .

JOURNAL OF BACTERIOLOGY, 1993, 175 (21) :6932-6938

[8] BROAD HOST RANGE DNA CLONING SYSTEM FOR GRAM-NEGATIVE BACTERIA - CONSTRUCTION OF A GENE BANK OF RHIZOBIUM-MELILOTI [J].

DITTA, G ;

STANFIELD, S ;

CORBIN, D ;

HELINSKI, DR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (12) :7347-7351

[9] PRECISE EXCISION AND INSTABILITY OF THE TRANSPOSON TN5 IN PSEUDOMONAS-AERUGINOSA [J].

GOLDBERG, JB ;

WON, J ;

OHMAN, DE .

JOURNAL OF GENERAL MICROBIOLOGY, 1990, 136 :789-796

[10] MOLECULAR-CLONING AND EXPRESSION OF THE BIODEGRADATIVE THREONINE DEHYDRATASE GENE (TDC) OF ESCHERICHIA-COLI-K12 [J].

GOSS, TJ ;

DATTA, P .

MOLECULAR & GENERAL GENETICS, 1985, 201 (02) :308-314