Human Gyrovirus-Apoptin Interferes with the Cell Cycle and Induces G2/M Arrest Prior to Apoptosis

被引：17

作者：

Chaabane, Wiem ^{[1
]}

Ghavami, Saeid ^{[2
,3
]}

Malecki, Andrzej ^{[4
]}

Los, Marek J. ^{[5
]}

机构：

[1] Tunis Univ, Dept Biol, Fac Sci, Tunis 2092, Tunisia

[2] Univ Manitoba, Dept Human Anat & Cell Sci, Fac Hlth Sci, Coll Med, Winnipeg, MB, Canada

[3] Shiraz Univ Med Sci, Hlth Policy Res Ctr, Shiraz, Iran

[4] Jerzy Kukuczka Acad Phys Educ Katowice, Fac Physiotherapy, Katowice, Poland

[5] Med Univ Silesia, ENT Dept, Sch Med, Katowice, Poland

来源：

ARCHIVUM IMMUNOLOGIAE ET THERAPIAE EXPERIMENTALIS | 2017年 / 65卷 / 06期

关键词：

Apoptin; PKTCs; Cell cycle; APC; Cyclin B1; ANAPHASE-PROMOTING COMPLEX/CYCLOSOME; STEM-CELLS; CANCER; DIFFERENTIATION; INHIBITION; AUTOPHAGY; NUCLEUS; PATHWAY; TARGET; AKT;

D O I：

10.1007/s00005-017-0464-8

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The human gyrovirus-Apoptin (HGyv-Apoptin) is a protein that gained attention because it is selectively cytotoxic toward cancer cells. In this study, we have investigated the effect of HGyv-Apoptin on cell cycle progression of cancer cells. We also compared HGyv-Apoptin's action to its homologue chicken anemia virus Apoptin (CAV-Apoptin). We show that HGyv-Apoptin induces G2/M arrest in cancer cells. This is at least in part due to the fact that HGyv-Apoptin induces an abnormal spindle formation in mitotic cells that do not progress properly throughout the cell cycle. HGyv-Apoptin most likely inhibits APC function leading to a sustained cyclin-B1-expression. These results indicate that HGyv-Apoptin has a similar mechanism of action as its homolog CAV-Apoptin and further supports its cancer therapeutic potential.

引用

页码：545 / 552

页数：8

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