Structural Changes in Bacteriorhodopsin in Response to Alternate Illumination Observed by High-Speed Atomic Force Microscopy

被引:38
|
作者
Shibata, Mikihiro [1 ]
Uchihashi, Takayuki [1 ]
Yamashita, Hayato [1 ]
Kandori, Hideki [2 ]
Ando, Toshio [1 ]
机构
[1] Kanazawa Univ, Dept Phys, Kanazawa, Ishikawa 920, Japan
[2] Nagoya Inst Technol, Dept Frontier Mat, Showa Ku, Nagoya, Aichi, Japan
关键词
chromophores; electron microscopy; membrane proteins; nanotechnology; protein structures; X-RAY-DIFFRACTION; PROTON TRANSLOCATION; ELECTRON CRYSTALLOGRAPHY; BIOMOLECULAR PROCESSES; ANGSTROM RESOLUTION; NANO-VISUALIZATION; SURFACE-STRUCTURES; N INTERMEDIATE; MEMBRANE; PHOTOCYCLE;
D O I
10.1002/anie.201007544
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Blue light, green light: High-speed atomic force microscopy visualized light-induced structural changes of the D96N bacteriorhodopsin (bR) mutant under alternate two-color illumination. With green light, each bR molecule is displaced outward from the trimer center. This activated structure is driven back to the ground state by the subsequent blue-light illumination (see picture). Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码:4410 / 4413
页数:4
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