Calpains are involved in asexual and sexual development, cell wall integrity and pathogenicity of the rice blast fungus

被引：12

作者：

Liu, Xiao-Hong ^{[1
]}

Ning, Guo-Ao ^{[1
]}

Huang, Lu-Yao ^{[1
]}

Zhao, Ya-Hui ^{[1
]}

Dong, Bo ^{[2
]}

Lu, Jian-Ping ^{[3
]}

Lin, Fu-Cheng ^{[1
]}

机构：

[1] Zhejiang Univ, Inst Biotechnol, State Key Lab Rice Biol, Hangzhou 310058, Zhejiang, Peoples R China

[2] Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou 310021, Zhejiang, Peoples R China

[3] Zhejiang Univ, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

基金：

中国国家自然科学基金;

关键词：

UBIQUITIN-PROTEASOME SYSTEM; MAGNAPORTHE-GRISEA; CYSTEINE PROTEASE; GENE; KINASE; GROWTH; ASPERGILLUS; ADAPTATION; DISRUPTION; INFECTION;

D O I：

10.1038/srep31204

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Calpains are ubiquitous and well-conserved proteins that belong to the calcium-dependent, non-lysosomal cysteine protease family. In this study, 8 putative calpains were identified using Pfam domain analysis and BlastP searches in M. oryzae. Three single gene deletion mutants (Delta Mocapn7, Delta Mocapn9 and Delta Mocapn14) and two double gene deletion mutants (Delta Mocapn4 Delta Mocapn7 and Delta Mocapn9 Delta Mocapn7) were obtained using the high-throughput gene knockout system. The calpain disruption mutants showed defects in colony characteristics, conidiation, sexual reproduction and cell wall integrity. The mycelia of the Delta Mocapn7, Delta Mocapn4 Delta Mocapn7 and Delta Mocapn9 Delta Mocapn7 mutants showed reduced pathogenicity on rice and barley.

引用

页数：12

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