Wheat vacuolar H+-ATPase subunit B cloning and its involvement in salt tolerance

被引：33

作者：

Wang, Li ^{[1
]}

He, Xiaoliang ^{[1
]}

Zhao, Yanjun ^{[1
]}

Shen, Yinzhu ^{[1
]}

Huang, Zhanjing ^{[1
]}

机构：

[1] Hebei Normal Univ, Coll Life Sci, Shijiazhuang, Hebei, Peoples R China

来源：

PLANTA | 2011年 / 234卷 / 01期

关键词：

Gene transformation; Salt tolerance; TaVB; V-H+-ATPase; V-ATPASE; OSMOTIC-STRESS; EXPRESSION; MEMBRANE; LEAVES; ADAPTATION; GROWTH; ROOTS; PLANT;

D O I：

10.1007/s00425-011-1383-2

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Wheat vacuolar H+-ATPases (V-ATPase) subunit B, named TaVB, was isolated from the salt-tolerant wheat RH8706-49 and used to transform Arabidopsis plants. TaVB-expressed Arabidopsis has a higher germination rate, root length, V-H+-ATPase activity, and overall salt tolerance than the wild type, indicating that expression of the gene can affect salt tolerance of the transgenic plants. Under salt stress, intracellular Na+ levels in transgenic plants are significantly lower than the control. These results suggest that expression of the wheat TaVB gene may enhance plant tolerance to salt stress.

引用

页码：1 / 7

页数：7

共 30 条

[21] Structure, function and regulation of the vacuolar (H+)-ATPase [J].

Stevens, TH ;

Forgac, M .

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 1997, 13 :779-808

[22] Salt regulation of transcript levels for the c subunit of a leaf vacuolar H+-ATPase in the halophyte Mesembryanthemum crystallinum [J].

Tsiantis, MS ;

Bartholomew, DM ;

Smith, JAC .

PLANT JOURNAL, 1996, 9 (05) :729-736

[23] Salt stress in Thellungiella halophila activates Na+ transport mechanisms required for salinity tolerance [J].

Vera-Estrella, R ;

Barkla, BJ ;

García-Ramírez, L ;

Pantoja, O .

PLANT PHYSIOLOGY, 2005, 139 (03) :1507-1517

[24] Effects of salt treatment and osmotic stress on V-ATPase and V-PPase in leaves of the halophyte Suaeda salsa [J].

Wang, BS ;

Lüttge, U ;

Ratajczak, R .

JOURNAL OF EXPERIMENTAL BOTANY, 2001, 52 (365) :2355-2365

[25]

Wang H, 1999, PROG BIOCHEM BIOPHYS, V26, P184

[26]

WANG YZ, 1993, ACTA BIOCH BIOPH SIN, V25, P481

[27] PROTON TRANSPORT ACTIVITY OF THE PURIFIED VACUOLAR H+-ATPASE FROM OATS - DIRECT STIMULATION BY CL- [J].

WARD, JM ;

SZE, H .

PLANT PHYSIOLOGY, 1992, 99 (03) :925-931

[28] Adaptation of H+-pumping and plasma membrane H+ ATPase activity in proteoid roots of white lupin under phosphate deficiency [J].

Yan, F ;

Zhu, YY ;

Müller, C ;

Zörb, C ;

Schubert, S .

PLANT PHYSIOLOGY, 2002, 129 (01) :50-63

[29] Functional proteomics; current achievements [J].

Yanagida, M .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2002, 771 (1-2) :89-106

[30] Cloning and functional analysis of wheat V-H plus -ATPase subunit genes [J].

Zhao, Qian ;

Zhao, Yan-Jun ;

Zhao, Bao-Cun ;

Ge, Rong-Chao ;

Li, Ming ;

Shen, Yin-Zhu ;

Huang, Zhan-Jing .

PLANT MOLECULAR BIOLOGY, 2009, 69 (1-2) :33-46

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