Wheat vacuolar H+-ATPase subunit B cloning and its involvement in salt tolerance

被引：33

作者：

Wang, Li ^{[1
]}

He, Xiaoliang ^{[1
]}

Zhao, Yanjun ^{[1
]}

Shen, Yinzhu ^{[1
]}

Huang, Zhanjing ^{[1
]}

机构：

[1] Hebei Normal Univ, Coll Life Sci, Shijiazhuang, Hebei, Peoples R China

来源：

PLANTA | 2011年 / 234卷 / 01期

关键词：

Gene transformation; Salt tolerance; TaVB; V-H+-ATPase; V-ATPASE; OSMOTIC-STRESS; EXPRESSION; MEMBRANE; LEAVES; ADAPTATION; GROWTH; ROOTS; PLANT;

D O I：

10.1007/s00425-011-1383-2

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Wheat vacuolar H+-ATPases (V-ATPase) subunit B, named TaVB, was isolated from the salt-tolerant wheat RH8706-49 and used to transform Arabidopsis plants. TaVB-expressed Arabidopsis has a higher germination rate, root length, V-H+-ATPase activity, and overall salt tolerance than the wild type, indicating that expression of the gene can affect salt tolerance of the transgenic plants. Under salt stress, intracellular Na+ levels in transgenic plants are significantly lower than the control. These results suggest that expression of the wheat TaVB gene may enhance plant tolerance to salt stress.

引用

页码：1 / 7

页数：7

共 30 条

[1] SOS2 promotes salt tolerance in part by interacting with the vacuolar H+-ATPase and upregulating its transport activity
Batelli, Giorgia
Verslues, Paul E.
Agius, Fernanda
Qiu, Quansheng
Fujii, Hiroaki
Pan, Songqin
Schumaker, Karen S.
Grillo, Stefania
Zhu, Jian-Kang
[J]. MOLECULAR AND CELLULAR BIOLOGY, 2007, 27 (22) : 7781 - 7790
[2] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[3] Chinnusamy Viswanathan, 2006, Genet Eng (N Y), V27, P141, DOI 10.1007/0-387-25856-6_9
[4] Floral dip:: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana
Clough, SJ
Bent, AF
[J]. PLANT JOURNAL, 1998, 16 (06) : 735 - 743
[5] Structural organization of the V-ATPase and its implications for regulatory assembly and disassembly
Diepholz, Meikel
Boersch, Michael
Boettcher, Bettina
[J]. BIOCHEMICAL SOCIETY TRANSACTIONS, 2008, 36 : 1027 - 1031
[6] Significance of the V-type ATPase for the adaptation to stressful growth conditions and its regulation on the molecular and biochemical level
Dietz, KJ
Tavakoli, N
Kluge, C
Mimura, T
Sharma, SS
Harris, GC
Chardonnens, AN
Golldack, D
[J]. JOURNAL OF EXPERIMENTAL BOTANY, 2001, 52 (363) : 1969 - 1980
[7] cDNA sequence and expression of subunit E of the vacuolar H+-ATPase in the inducible Crassulacean acid metabolism plant Mesembryanthemum crystallinum
Dietz, KJ
Arbinger, B
[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1996, 1281 (02): : 134 - 138
[8] The vacuolar H+-ATPase: A universal proton pump of eukaryotes
Finbow, ME
Harrison, MA
[J]. BIOCHEMICAL JOURNAL, 1997, 324 : 697 - 712
[9] Cloning and functional characterization of a wheat serine/threonine kinase gene (TaSTK) related to salt-resistance
Ge, Rong-Chao
Chen, Gui-Ping
Zhao, Bao-Cun
Shen, Yin-Zhu
Huang, Zhan-Jing
[J]. PLANT SCIENCE, 2007, 173 (01) : 55 - 60
[10] Salt-induced expression of the vacuolar H+-ATPase in the common ice plant is developmentally controlled and tissue specific
Golldack, D
Dietz, KJ
[J]. PLANT PHYSIOLOGY, 2001, 125 (04) : 1643 - 1654

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