Functional characterization of alpha-synuclein protein with antimicrobial activity

被引：69
作者：
Park, Seong-Cheol [1 ]
Moon, Jeong Chan [2 ,3 ]
Shin, Su Young [2 ]
Son, Hyosuk [4 ]
Jung, Young Jun [2 ]
Kim, Nam-Hong [1 ]
Kim, Young-Min [1 ]
Jang, Mi-Kyeong [1 ]
Lee, Jung Ro [2 ]
机构：
[1] Sunchon Natl Univ, Dept Polymer Sci & Engn, Sunchon 57922, Jeollanam Do, South Korea
[2] Natl Inst Ecol, 1210 Geumgang Ro, Maseo Myeon 33657, Seocheon Gun, South Korea
[3] Busan Inst Natl Forens Serv, DNA Anal Sect, 50 Kumoh Ro, Yangsan Si 50612, Gyeongsangnam D, South Korea
[4] Natl Marine Biodivers Inst Korea, 75 Jangsan Ro, Janghang Eup 33662, Seocheon Gun, South Korea
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2016年 / 478卷 / 02期
关键词：
Alpha-synuclein; Antibacterial activity; Antifungal activity; Defense mechanism; PARKINSONS-DISEASE; PLASMA-MEMBRANE; BINDING; AGGREGATION; PEPTIDES; BRAIN;
D O I：
10.1016/j.bbrc.2016.08.052
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Alpha-synuclein (alpha-Syn), a small (14 kDa) protein associated with Parkinson's disease, is abundant in human neural tissues. alpha-Syn plays an important role in maintaining a supply of synaptic vesicles in presynaptic terminals; however, the mechanism by which it performs this function are not well understood. In addition, there is a correlation between alpha-Syn over-expression and upregulation of an innate immune response. Given the growing body of literature surrounding antimicrobial peptides (AMPs) in the brain, and the similarities between alpha-Syn and a previously characterized AMP, Amyloid-beta, we set out to investigate if alpha-Syn shares AMP-like properties. Here we demonstrate that alpha-Syn exhibits antibacterial activity against Escherichia coli and Staphylococcus aureus. In addition, we demonstrate a role for cc-Syn in inhibiting various pathogenic fungal strains such as Aspergillus flavus, Aspergillus fumigatus and Rhizoctonia solani. We also analyzed localizations of recombinant alpha-Syn protein in E. coli and Candida albicans. These results suggest that in addition to alpha-Syn's role in neurotransmitter release, it appears to be a natural AMP. (C) 2016 Elsevier Inc. All rights reserved.
引用
收藏
页码：924 / 928
页数：5
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