Over expression of a cytochrome p450 (CYP6P9) in a major African malaria vector, Anopheles funestus, resistant to pyrethroids

被引：109

作者：

Amenya, D. A. ^{[1
,2
]}

Naguran, R. ^{[1
]}

Lo, T. -C. M. ^{[1
]}

Ranson, H. ^{[4
]}

Spillings, B. L. ^{[1
,2
,3
]}

Wood, O. R. ^{[1
,2
,3
]}

Brooke, B. D. ^{[1
,2
,3
]}

Coetzee, M. ^{[1
,2
,3
]}

Koekemoer, L. L. ^{[1
,2
,3
]}

机构：

[1] NHLS, Natl Inst Communicable Dis, Vector Control Reference Unit, ZA-2131 Johannesburg, South Africa

[2] Univ Witwatersrand, Sch Anim Plant & Environm Sci, Johannesburg, South Africa

[3] Natl Hlth Lab Serv, Sch Pathol, Div Virol & Communicable Dis Surveillance, Johannesburg, South Africa

[4] Univ Liverpool, Liverpool Sch Trop Med, Vector Grp, Liverpool L3 5QA, Merseyside, England

来源：

INSECT MOLECULAR BIOLOGY | 2008年 / 17卷 / 01期

关键词：

P450; insecticide resistance; mosquito;

D O I：

10.1111/j.1365-2583.2008.00776.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Anopheles funestus Giles is one of the major African malaria vectors. It has previously been implicated in a major outbreak of malaria in KwaZulu/Natal, South Africa, during the period 1996 to 2000. The re-emergence of this vector was associated with monooxygenase-based resistance to pyrethroid insecticides. We have identified a gene from the monooxygenase CYP6 family, CYP6P9, which is over expressed in a pyrethroid resistant strain originating from Mozambique. Quantitative Real-Time PCR shows that this gene is highly over expressed in the egg and adult stages of the resistant strain relative to the susceptible strain but the larval stages showed almost no difference in expression between strains. This gene is genetically linked to a major locus associated with pyrethroid resistance in this A. funestus population.

引用

页码：19 / 25

页数：7

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