Saccharopolyspora lacisalsi sp nov., a novel halophilic actinomycete isolated from a salt lake in Xinjiang, China

被引:17
作者
Guan, Tong-Wei [1 ,2 ]
Wu, Nan [3 ]
Xia, Zhan-Feng [2 ]
Ruan, Ji-Sheng [2 ]
Zhang, Xiao-Ping [4 ]
Huang, Ying [2 ]
Zhang, Li-Li [1 ]
机构
[1] Tarim Univ, Key Lab Protect & Utilizat Biol Resources Tarim B, Alar 843300, Xinjiang, Peoples R China
[2] Chinese Acad Sci, State Key Lab Microbial Resources, Inst Microbiol, Beijing 100101, Peoples R China
[3] Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Key Lab Biogeog & Bioresource Arid Land, Urumqi 830011, Peoples R China
[4] Sichuan Agr Univ, Fac Resource & Environm Sci, Yaan 625000, Peoples R China
基金
中国国家自然科学基金;
关键词
Saccharopolyspora lacisalsi sp nov; Halophilic; Salt lake; GENUS SACCHAROPOLYSPORA; DEOXYRIBONUCLEIC-ACID; CORYNEFORM BACTERIA; GOODFELLOW; 1975; FATTY-ACID; IDENTIFICATION; CLASSIFICATION; MEMBER; LACEY; SOIL;
D O I
10.1007/s00792-011-0369-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A novel actinomycete strain, designated TRM 40133(T), was isolated from a hypersaline habitat of Tarim basin in Xinjiang Province, north-west China. Its taxonomic status was determined using a polyphasic approach. Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence of the strain showed that it formed a well-seperated sub-branch within the radiation of the genus Saccharopolyspora. The highest levels of 16S rRNA gene sequence similarity was found between the strain TRM 40133(T) and Saccharopolyspora qijiaojingensis YIM 91168(T) (96.5%). The chemotaxonomic characteristics of the isolate are typical for the genus Saccharopolyspora. It contained meso-DAP as the diagnostic diamino acid. Whole cell hydrolysate contained arabinose, xylose, ribose and glucose. The diagnostic phospholipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol and two unknown phospholipids. The main menaquinone was MK-9(H-6) and MK-9(H-4). No mycolic acid was detected. The predominant cellular fatty acids were iso-C-16:0 and anteiso-C-17:0. The G+C content of the genomic DNA was 68.2 mol%. In addition, the strain TRM 40133(T) had a phenotypic profile that readily distinguished it from the recognized representatives of the genus Saccharopolyspora. The strain TRM 40133(T) therefore represents a novel species of the genus Saccharopolyspora, for which the name Saccharopolyspora lacisalsi sp. nov. is proposed. The type strain is TRM 40133(T) (=KCTC 19987(T) =CCTCC AA 2010012(T)).
引用
收藏
页码:373 / 378
页数:6
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